بررسي تنوع فنوتيپي و ژنوتيپي گونه هاي pectobacterium عامل پوسيدگي نرم، جدا شده از ميزبان هاي مختلف در برخي مناطق ايران

Phenotype and genotype diversity of soft rot pectobacteria isolated from different plant hosts in several regions of Iran

Pectobacterium is one of the major destructive causal agents in most crop plants throughout the world. During the years 2007-2008, 38 isolate of soft rot pectobacteria were isolated from different hosts including: potato, onion, cabbage and carrot in Hamedan, Semnan, Hormozgan, Kerman, East Azarbaijan, Khuzestan, Zanjan, Tehran and Isfahan provinces. Phenotypic identification of the strains were performed using recommended biochemical and physiological tests. Also genetic diversity determined by BOX-PCR using BOX AIR primer. According to results, all strains identified as "Carotovora" group as Pectobacterium carotovorum subsp. carotovorum, P. atrosepticum and Dickeya chrysanthemi. Also some characteristics of the pathogen differ from those described for this bacterium isolated in some countries. The combined results of performed tests showed a high variability among pectolytic bacteria in Iran and there was no clear relationship between the resulting DNA fingerprints and geographical origins of isolates.