بررسى توانايى اتصال لاكتوباسيلوس هاى جدا شده از محصولات لبنى تخميرى سنتى به لالين سلولى CaCO2 با دو روش كشت و رنگ آميزي گرم

Evaluation of adhesion ability of lactobacilli isolated from traditional fermented dairy products to CaCO2 cell line by culture and gram staining methods

The aim of this study was to asses adhesion ability of lactobacilli isolated from traditional fermented dairy products to epithelial cells of human GI tract and in comparison to trade well adhere strain La-5. In previous study probiotic potential of these strains like resistance to acidic pH and bile salt had been proved. In this study adhesion ability of these strains were assed by culture and gram staining. Methods A1a1, Y2l6 in gram staining method and Y2l6, C6l2, A1a1 and C6c4 in culture method showed very well adhesion like La-5. These results demonstrated that culture method is more confidente than gram staining method. In conclusion it seems that Y2l6, C6l2, A1a1 and C6c4 have GIT colonization ability. Of course these strains require further invitro and invivo investigations to introduce as Iranian native probiotic bacteria who can compete in functional properties with trade strains.