توليد ايمونوگلوبولين در زرده تخم مرغ عليه پروتئين UreC هليكوباكتر پيلوري به وسيله واكسيناسيون DNA

Production of chicken egg yolk immunoglobulin against Helicobacter pylori UreC protein by DNA vaccination

Aims: Current therapies against Helicobacter pylori infection, which is associated with gastritis, peptic ulcer and gastric cancer, are performed by antibiotic use. Due to increase of antibiotic resistance and difficulty and cost of treatment, new approaches have focused on using specific antibodies. Accordingly, efficiency of antibodies specific to H. pylori urease has been demonstrated. Considering the longtime benefit and efficacy of DNA vaccination, this study was designed on order to use DNA vaccination for generation of egg yolk immunoglobulin against UreC subunit of H. pylori urease. Materials & Methods: H. pylori ureC gene was cloned into eukaryotic pCI expression vector. Recombinant pCI-ureC plasmid, amplified in E. coli host were purified and used for genetic immunization of chickens. IgY recovered from egg yolk, using Poly Ethylene Glycol precipitation and analyzed by indirect ELISA and urease test Results: Using DNA vaccination, specific and biologically active IgY antibodies were produced and extracted, which based on ELISA results is able to detect UreC subunit of urease. Conclusion: DNA immunization can be used as a productive and economic method to generate efficient anti- urease antibodies for therapeutic proposes. Keywords: Helicobacter pylori, Egg Yolk Immunoglobulin (IgY), Urease, DNA Vaccine