بررسي بيان پروتئين هاي p53 و HER2 با عوامل مرتبط با پيش آگهي كارسينوم معده به روش ايمونوهيستوشيمي

Determining the expression of p53 and HER2 with the related factors in prognosis of gastric carcinoma by immunohistochemistry

Background and purpose: Gastric carcinoma is one of the most common malignancies and its early diagnosis can be effective in the treatment method and the rate of the patientsʹ survival. In the past recent years, many genes have been identified which may have relationship with the prognosis of this disease and the patientʹs survival. This study is going to investigate the rate and pattern of p53 and HER2 expression in gastric carcinoma by immunohistochemistry (IHC). Materials and methods: 114 paraffin wax-embedded gastric carcinoma specimens were selected and the results obtained from IHC stains were compared with histopathologic findings. The data were analyzed using Chi Square test in SPSS. Results: In this study, male to female ratio was 1.92 with the average age of 65.44 ± 10.92. The most common tumor locations were antrum or pylorus (54 cases) and the average size of tumor was 5.32 ± 2.62 cm. According to Lauren classification, intestinal tumor (101 cases) and in Ming classification, infiltrative tumor (74 cases) were the most prevailing. The most dominant cases were grade 2 (58 cases) and T3 staging (76 cases). Lymph nodes metastasis was also observed in 70 cases. The amount of p53 and HER2 expression were 52.6% and 24.6% of gastric carcinomas, respectively, showing a significant correlation between these two (P<0.05). Also a significant correlation (P<0.05) was found between the rate of p53 expression and tumor size, location of tumor and lymph node metastasis (P=0.05) in gastric cancers. Besides, HER2 protein had significant correlation with age of patient, tumor size, and Laurenʹs classification intestinal type carcinoma (P<0.05). Conclusion: The results of the study indicated that the incidence of p53 protein is associated with larger tumor size and lymph node metastasis. HER2 protein and its expression in gastric cancer are similar to ductal adenocarcinoma in breast cancer.