مطالعه هيستوشيمي غدد برونر در گاو ميش هاي استان خوزستان

Histochemical study of brunners gland in buffalo of Khuzestan province

The study was ai,med to demonstrate the distribution, morphological properties of Brunners glands in the small intestine of the buffalo. The duodenum of 14 healthy animals of both sexes constituted the material of the study. The glands were compoposed of acini densely packed within the submucosa. The brunnrers glands contained two types of cells, namely, serous and mucous cells.histochemical examination revealed that the mucous glands and secretory ducts did not react with the periodic acid Schiff stain, while serous glands were weakly pas-positive. Furthermore, mucous glands reacted positively with alcian blue ph 2.5. when applied the combined aldehyde fuchin-alcian blue ph 2.5 staining procedure, mucous glands were determined to be aldehyde fuchsin(-) and alcian blue(+). These results showed that while a limited amount of neutral carbohydrates was secreted in serous glands. The secretion of the ducts and mucous cells of the duodenal glands in the buffalo was composed of acidic carbohydrates whit this acidity being due to the presence of carboxyl groups.males and females did not differ in the histochemical staining properties of the duodenal secretion.