كليدواژه :
بابونه , التيام زخم , خوكچه هندي , هيستولوژي
چكيده لاتين :
To improve wound healing, Medicinal herb utilization has been always one of the major subjects of interest in medicine. In order to evaluate the effect (s) of Matricaria chamomilla on cutaneous wound healing in guinea pig histologically, this study was designed. To do this, 60 male guinea pigs were purchased and randomly divided into three groups including experimental, placebo, and control. Then, each group was subdivided into four subgroups based on defined periods (days 3, 5, 7, 14 after experiment initiation), each contained five guinea pigs .Animals were anesthetized with intraperitoneal (IP) injection of Ketamine hydrochloride and Xylazine. 2x2 centimeters full-thickness excisions were made on the skin of each guinea pig in right side of the back region. Experimental and placebo groups received treatment with Matricaria chamomilla ointment and Vaseline, respectively. The treatments were continued twice a day till euthanasia on the above mentioned periods. Control group received no treatment. Animals were euthanatized with intracardiac injection of Ketamine hydrochloride on the defined periods. Specimens were taken from the wounded areas and collected in 10% formalin solution. The specimens were processed and sectioned for 5 micrometers. Sections were stained with Hematoxylin & Eosin and studied microscopically. Finally, the results were analyzed statistically. Histologic study showed significant differences (P < 0.05) in extent of inflammation (days 7) and intensity of inflammation (days 5 and 7) among the groups. Results of this study indicated that Matricaria chamomilla could improve wound healing through reducing extent and intensity of inflammation.
