مطالعه اثرات هيدروكسي آپاتيت در مدل تاخيري التيام شكستگي در استخوان زند زبرين موش صحرايي

Study of the effects of Hydroxy apatite on delayed Radial Fracture Healing in Rat

Hydroxyapatite (HA), as bone substitute materials has the advantages of abundant supply and absence of immunogenecity. This study was conducted to evaluate the effects of hydroxyapatite on delayed radial fracture healing in rats was assessed by radiographic and histological methods. Eighteen healthy rats were randomly divided into two equal groups. Each group was divided to three subgroups (n = 3). All rats were generally anesthetized and transverse osteotomy was performed in the mid-shaft of the right radius under aseptic condition. In group 1, the osteotomy gap was filled with the HA. Group 2 not received any treatment, and was used as a negative control. Radiographs were taken in lateral view at days of 0, 14, 28 and 42. From each subgroup three rats were euthanized at days of 14, 28 and 42 and the radial bones harvested and prepared for histological test. In radiography, callus formation, gap and cross reaction between radius and ulna were evaluated. In histology, healing rate and tissue inflammation were evaluated. Radiographic signs of bone healing in negative control were lower than from treated group. In histological finding, healing process in treated group was higher than from negative control significantly. It was concluded that application of hydroxyapatite has the positive effects on delayed radial fracture healing process in rats.