پديد آورندگان :
ديانت، م. نويسنده Department of Agronomy and Plant Breeding, Agricultural and Natural Resources Campus, University of Tehran, Karaj Dianat, .M , شاه نجات بوشهري، ا. ا. نويسنده Department of Agronomy and Plant Breeding, Agricultural and Natural Resources Campus, University of Tehran, Karaj Shahnejat Booshehri, .A .A , عليزاده، ح. م. نويسنده Faculty of Agriculture, University of Tehran, Karaj Alizadeh, .H .M , نقوي، م. ر. نويسنده Department of Agronomy and Plant Breeding, Agricultural and Natural Resources Campus, University of Tehran, Karaj Naghavi, .M .R , رحيميان مشهدي، ح. نويسنده Faculty of Agriculture, University of Tehran, Karaj Rahimian-Mashhadi, .H
كليدواژه :
RAPD markers , genetic diversity , Phragmites australis , Common reed
چكيده فارسي :
ني از علف هاي هرز چندساله است كه وسيع ترين پراكنش را در بين گياهان گلدار دنيا دارد. 39 جمعيت ني در ايران جمع آوري شد و تنوع ژنتيكي بين آنها با استفاده از نشانگرهاي RAPD بررسي شد. 16 پرايمر 149 باند توليد كردند كه 82% آن يعني 123 باند، پلي مورفيك بود. ضرايب شباهت ژنتيكي بين جمعيت ها از 44/0تا 84/0 متغير و متوسط شباهت ژنتيكي 60/0 بود. نتايج آزمايش نشان داد كه نشانگرهاي RAPD براي بررسي تنوع ژنتيكي ني، مناسب بودند. هچنين شباهت ژنتيكي بين جمعيت ها اهميت تكثير رويشي را در اين علف هرز نشان مي دهد.
چكيده لاتين :
The common reed (Phragmites australis) is a perennial weed with the largest geographical distribution of any flowering plant in the world. In this study, 39 populations of common reed were collected and random amplified polymorphic DNA (RAPD) markers were used to characterize the genetic diversity among these populations. The 16 primers used in this study, amplified 149 scorable RAPD bands among which 123 were polymorphic (82%). Genetic similarity coefficients ranged from 0.44 to 0.84, with an average of 0.60. Results showed that RAPD markers are suitable for the evaluation of genetic diversity of common reed. The results support the clonal nature of P.australis with its growth and spread primarily by vegetative propagation.
