چكيده فارسي :
ميزان گلوكز سرم به عنوان شاخص پيش آگهي دهنده در برخي از بيماري هاي اسب نظير كوليك ، هيپو گليسمي ، سپتي سمي ، اسهال ، ديابت مليتوس و كوشينگ مطرح مي باشد. با توجه به ماهيت اورژانسي اختلالات فوق الذكر، اندازه گيري سريع و دقيق اين پارامتر ضروري است . دستگاه هاي گلوكومتر رايج در بازار، همگي از نوع انساني بوده و با توجه به رقابت بالايي در اين بخش، مطالعات متعددي جهت بررسي كارآيي اين دستگاه ها انجام پذيرفته است. متاسفانه در بخش طب دلمپزشكي مطالعات چنداني انجام نشده است . هدف از اين مطالعه ارزيابي اعتبار سنجي دستگاه گلوكومتر EasyGluco جهت اندازه گيري ميزان گلوكز خون و استفاده باليني از آن در طب باليني اسب مي باشد . تعداد 45 راس اسب خون گرم از اسب هاي سالم و بيمار (بستري در بيمارستان اسب داري) با شرايط سني مختلف انتخاب شدند.از هر راس دام ميزان 3 سي سي خون از طريق وريد وداج اخذ و به لوله حاوي ماده ضد انعقاد فلورور سديم منتقل مي گرديد . بلافاصله در همان محل ، مقادير گلوكز با دستگاه گلوكومترEasygluco از نمونه هاي خون مويرگي و وريدي تعيين مقدار و ثبت مي گرديد . پلاسما در اسرع وقت جدا و با حفظ زنجيره سرما به آزمايشگاه منتقل و تا زمان آزمايش در 20- درجه سانتي گراد نگهداري گرديد . ميزان گلوكز پلاسما با روش فوتومتري و با استفاده از كيت شركت پارس آزمون تعيين گرديد. جهت بررسي تكرارپذيري، دقت و Within run imprecision دستگاه گلوكومتر ، 1 نمونه خون براي 15 بار متوالي و از فواصل 15 ثانيه تكرار و ميزان C.V. تعيين و با C.V. نمونه هاي كنترل مقايسه شد. ميزان CV در نمونه خون اخذ شده ، كنترل هاي پايين و بالا به ترتيب 4.1 ،3.3 و 4.4 درصد بود كه همگي با مقادير NCCLSهم خواني داشت .
چكيده لاتين :
Serum glucose level considered as a prognostic indicator in a
variety of equine diseases such as colic , hypoglycemic conditions,
septicemia, diarrhea, diabetes mellitus. Considering the emergency
nature of disorders mentioned above, fast and accurate measurement of
this parameter is necessary. Self-monitoring of blood glucose (SMBG)
allows diabetic patients to achieve and maintain specific glycaemic
goals. There are several glucometer devices in the market, all of human
kind and thereʹs high competition between companies .,several studies
has performed to evaluate efficacy of this device in medicine.
Unfortunately limited studies has been done in veterinary medicine .
The aim of this study was to evaluate validity of validation EasyGluco
glucometer to measure blood glucose levels and itʹs application in
equine clinical practice. Fourthy five horses warm blooded healthy and
sick horses (horses in the hospital) with different ages were selected . 3
ml of blood were collected in the tube containing sodium fluoride
anticoagulant .Glucose concentration of capillary and venous blood
samples were determined Immediately with glucometer Plasma
immediately separated and transferred , on ice to the laboratory & kept
at - 20 ° C until laboratory examination . The level of plasma glucose
were determined by photometric method using Pars Azmoon kit. To
evaluate repeatability, accuracy and Within run imprecision of
Easygluco glucometer , one sample were analyzed for 15 consecutive
time at intervals of 15 seconds and CV were determine & were
compared with control samples. CV in blood samples taken, low and
high controls were 4.1, 3.3 and 4.4 percent respectively in which all
were acceptable with NCCLS standards. In order to Evaluate the
diversion of glucometer results with standard methods, Bland - Altman
Plot was performed. Bias levels in venous and capillary glucometer
methods with standard methods, respectively, were -9.4 and -34.8 . To
determine the correlation between the results of the glucometer and
laboratory method , correlation coefficient determined. In this study,
the correlation coefficient (r) between photometer method and venous
blood glucometer device was 0.823. The results of this study revealed
lower correlation compare to human studies .It Seems that it might be
due high level of hematocrite in the horse & human calibrated device .
In order to overcome this problem , Correction Factor(CF) should be
calculate. CF obtained in this study In this study WAS 1.1. Th results of
this study indicates that correction factor should be applied , then after
glucometer results can be trusted.
