شناسايي نماتدهاي انگل گياهي ريزوسفر مركبات شرق گيلان و غرب مازندران

Identification of Plant Parasitic Nematodes of Citrus Orchards in Guilan eastand Mazandaran West Province

به منظور شناسايي نماتدهاي انگل گياهي مركبات شرق گيلان و غرب مازندران طي سال هاي 1387 و 1388 تعداد 110 نمونه خاك و ريشه از باغ هاي مركبات جمع آوري گرديد. پس از انتقال نمونه ها به آزمايشگاه، نماتدها استخراج، تثبيت و به گليسيرين منتقل شدند. سپس از نماتد هاي جدا شده به تفكيك جنس، اسلايدهاي ميكروسكوپي دايم تهيه شد. پس از بررسي هاي ميكروسكوپي مشخصات ريخت شناسي و ريخت سنجي تعيين و با استفاده از منابع معتبر علمي شناسايي انجام گرديد. در اين مطالعه 27 گونه متعلق به 15 جنس شامل: Aphelenchoides asterocaudatus, A. bicaudatus, A. sacchari, Aphelenchus avenae, Basiria graminophila, Boleodorus thylactus ,Criconemoides xenoplax, C. curvatum, Ditylenchus myceliophagus, Filenchus facultativus, F. vulgaris, Helicotylenchus digonicus , H. dihystera, H. exallus, H. pseudorobustus, H. vulgaris, Nagelus obscurus, Ogma civellae, Paratylenchus nanus, Pratylenchus loosi, P. neglectus, P. jaehni, P. zea, Psilenchus hilarulus, Seinura lii, S.prospera, Tylenchulus semipenetrans. شناسايي گرديد. گونه هايPratylenchus jaehni ، Seinura lii و S. prospera براي اولين بار از ايران گزارش مي شوند.

In order to identify the plant parasitic nematodes of citrus orchards in Guilan east and Mazandaran west province, 110 soil and root samples were collected during 2008 and 2009. The samples were washed and the nematodes extracted by centrifugal flotation technique. Then, they were fixed and transferred to glycerin. The permanent slides were prepared from the extracted nematodes. After microscopic consideration, the useful measurements and drawing were attained by using a drawing tube attached to the microscope. In this study 27 species belonging to 15 genera were identified using morphological and morphometrical characters as following: Aphelenchoides asterocaudatus, A. bicaudatus, A. sacchari, Aphelenchus avenae, Basiria graminophila, Boleodorus thylactus, Criconemoides xenoplax, C. curvatum, Ditylenchus myceliophagus, Filenchus facultativus, F. vulgaris, Helicotylenchus digonicus , H. dihystera, H. exallus, H. pseudorobustus, H. vulgaris, Nagelus obscurus, Ogma civellae, Paratylenchus nanus, Pratylenchus loosi, P. neglectus, P. jaehni, P. zea, Psilenchus hilarulus, Seinura lii, S.prospera , Tylenchulus semipenetrans. The species "Pratylenchus Jaehni, Seinura lii and S. prospera" which are reported for the first time in Iran.