پديد آورندگان :
Sakhravi، Ahmad نويسنده Department of Microbiology, Faculty of Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran Sakhravi, Ahmad , Esmail، Asli نويسنده Department of Microbiology, Faculty of Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran Esmail, Asli , Harzandi، Naser نويسنده Department of Microbiology, Faculty of Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran Harzandi, Naser , Feizabadi، Mohammadmehdi نويسنده Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran Feizabadi, Mohammadmehdi , Movahedi، Abdolreza نويسنده Department of Microbiology, Razi Vaccine & Serum Research Institute, Tehran University of Medical Sciences, Tehran, Iran Movahedi, Abdolreza , Mosavari، Nader نويسنده Department of Microbiology, Razi Vaccine & Serum Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mosavari, Nader , Sadeghi، Davoud نويسنده Master of Biophysics, Razi Vaccine & Serum Research Institute Sadeghi, Davoud
چكيده لاتين :
Background: Clostridium tetani or Nicolaier’s bacillus is an obligatory anaerobic, Gram-positive, movable with terminal or sub terminal spore. The chromosome of C. tetani contains 2,799,250 bp with a G+C content of 28.6%. The aim of this study was identification and genomic fingerprinting of the vaccine strain of C. tetani.
Materials and Methods: The vaccine strain of C. tetani was provided by Razi Vaccine and Serum Research Institute. The seeds were inoculated into Columbia blood agar and grown for 72 h and transferred to the thioglycolate broth medium for further 36 h culturing. The cultures were incubated at 35?C in anaerobic conditions. DNA extraction with phenol/ chloroform method was performed. After extraction, the consistency of DNA was assayed. Next, the vaccine strain was digested using pvuII enzyme and incubated at 37?C for overnight. The digested DNA was gel-electrophoresed by 1% agarose for a short time. Then, the gel was studied with Gel Doc system and transferred to Hybond N+membrane using standard DNA blotting techniques.
Results: The vaccine strain of C. tetani genome was fingerprinted by RFLP technique. Our preliminary results showed no divergence exists in the vaccine strain used for the production tetanus toxoid during the periods of 1990-2011.
Conclusion: Observation suggests that there is lack of significant changes in RFLP genomic fingerprinting profile of the vaccine strain. Therefore, this strain did not lose its efficiency in tetanus vaccine production. RFLP analysis is worthwhile in investigating the nature of the vaccine strain C. tetani.
