تاثير مصرف داروي آتورواستاتين بر بافت پانكراس موش صحرايي نر

The Effect of Atorvastatin on the Pancreatic Tissue of Male Rats

زمينه و هدف: استاتين‌ها داروهاي بازدارنده آنزيم هيدروكسي متيل گلو تاريل كوآنزيم Aمي‌باشند كه علاوه بر كاهش غلظت كلسترول پلاسما، كلسترول با دانسيته كم و تري‌گليسريد را نيز كاهش مي‌دهند. هدف اين مطالعه بررسي تاثير مصرف داروي آتورواستاتين بر بافت پانكراس موش صحرايي نر بود. روش بررسي: در اين مطالعه تجربي 32 سر موش صحرايي بالغ نر به چهار گروه مساوي؛ كنترل و سه گروه آزمون تقسيم شدند. گروه‌هاي آزمون به مدت 45 روز تحت درمان با داروي آتورواستاتين با دوزهاي 10،20 و30 ميلي‌گرم قرار داده شدند، بعد از اتمام دوره درمان موش‌ها با خونگيري از قلب كشته شده و بافت پانكراس آنها جدا شد. بعد از فيكس كردن، رنگ آميزي بافت‌ها و تهيه اسلايد، قطر جزاير لانگرهانس اندازه‌گيري شد و تعداد سلول‌هاي جزاير لانگرهانس شمارش شد. داده‌ها با آزمون آماري تي تجزيه و تحليل شدند. يافته‌ها: بين ميانگين قطر جزاير لانگرهانس در گروهاي آزمون و كنترل اختلاف معني‌داري وجود نداشت. ميانگين تعداد سلول‌هاي جزاير لانگرهانس در گروه‌هاي آزمون نسبت به كنترل افزايش يافت(05/0 > p) . نتيجه‌گيري: تجويز آتورواستاتين سبب افزايش تعداد سلول‌هاي جزاير لانگرهانس مي‌شود و افزايش سلول ها نيز سبب افزايش ترشح انسولين شده و در نتيجه قند خون كاهش مي‌يابد.

Background & aim: Statins are inhibitors drugs of hydroxymethyl glotaryl coenzyme A. In addition to decreasing plasma cholesterol, low-density cholesterol they reduce the rate of triglycerides. The aim of this study was to investigate the effect of atorvastatin on rat pancreatic tissue.In addition to decreasing the rate of plasma cholesterol concentration; they also decrease the rate of LDL and Triglyceride. The aim of this study was to investigate the effect of atorvastatin on rat pancreatic tissue. Methods: In this study, 32 adult male rats were divided into four groups, control and three test groups. To conduct the present study, 32 male adult rats were divided into 4 equal groups: one control group and three test groups. Then the test groups were treated by Atorvastatin for 45 days with 10mg (group1), 20mg (group2) and 30 mg (group3) doses respectively. At the end of the treatment period, all rats were killed by taking blood from their hearts. The pancreas tissue of each rat (N=32) was separated. After fixation, tissue preparation and slide staining, Longerhans diameter was measured and number of cells was counted. Data were analyzed by t test.After fixing and coloring the tissue, all slides were prepared and evaluated under the microscope the diameter of Iʹs of Longerhans and the number of cells. The data was analyzed through t-test. Results: There was no significant difference in the diameter mean of Longerhans between the test groups and the control group. The mean number of islets cells were increased in compared to the control group (P > 0.05).There was also a significant increase was shown in the number of Longerhans cells in the test groups in relation to the control group (p (05/0 > .(p > 0.05). Conclusion: The consumption of the atorvastatine causes an increase in the number of Longerhans cells. In addition, the increase in the number of cells also increases the insulin secretion. As a result, blood sugar is decreased. Key Words: Atorvastatin, Pancreas, Rat