شماره ركورد :
610227
عنوان مقاله :
Influence of KNO3, CaCl2 and MgSO4 Concentrations on Growth and Cichoric Acid Accumulation in Hairy Root Culture of Purple Coneflower (Echinacea purpurea L.)
عنوان فرعي :
Influence of KNO3, CaCl2 and MgSO4 Concentrations on Growth and Cichoric Acid Accumulation in Hairy Root Culture of Purple Coneflower (Echinacea purpurea L.)
پديد آورندگان :
Abdoli ، M نويسنده Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran Abdoli , M , Moieni ، A نويسنده Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran Moieni , A , Naghdi Badi ، H نويسنده Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Naghdi Badi , H
اطلاعات موجودي :
فصلنامه سال 1392 شماره 45
رتبه نشريه :
علمي پژوهشي
تعداد صفحه :
10
از صفحه :
75
تا صفحه :
84
كليدواژه :
Macro-elements , Cichoric acid , Fresh weight , Hairy roots , Echinacea purpurea L.
چكيده لاتين :
Background: Purple coneflower (Echinacea purpurea L.) is one of the most important medicinal plants. Hairy root culture of Echinace, represent a valuable alternative to field cultivation for the production of bioactive secondary metabolites. Objective: The present study investigates the effects of KNO3, CaCl2 and MgSO4 concentrations on growth and the biosynthesis of cichoric acid in Echinacea purpurea hairy root culture. Methods: Agrobacterium rhizogenes strain R15834 was used for hairy root induction. The experiment was performed with 0.5 g of fresh hairy roots (about 2 cm long segments) per 100 ml Erlenmeyer flasks containing 30 ml of hormone-free liquid 1/2MS salts with B5 vitamins supplemented with different amounts of KNO3 (3131, 3939, and 4747 mg/l), CaCl2 (85, 170, and 340 mg/l) and MgSO4 (370 (control), 600, and 1600 mg/l). After 4 weeks, growth parameters and cichoric acid amounts were assessed. Results: Results indicated that the highest fresh (99.69 g/l) and dry weight (6.91 g/l) of hairy roots were achieved using 3131 mg/l KNO3. Fresh and dry weights of hairy roots in various CaCl2 and MgSO4 concentrations were not significantly different from those of control. The highest amount of cichoric acid (30.55 mg/g DW) was produced by 1600 mg/l MgSO4 treated hairy roots after 4 weeks. Conclusion: The present work demonstrated the effectiveness of modified 1/2MS salts supplemented with B5 vitamins medium for improving hairy root growth and in vitro production of cichoric acid.
سال انتشار :
1392
عنوان نشريه :
گياهان دارويي
عنوان نشريه :
گياهان دارويي
اطلاعات موجودي :
فصلنامه با شماره پیاپی 45 سال 1392
كلمات كليدي :
#تست#آزمون###امتحان
لينک به اين مدرک :
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