پديد آورندگان :
Naghsh، Nooshin نويسنده Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Naghsh, Nooshin , Doudi، Monir نويسنده Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Doudi, Monir , Nikbakht، Zohreh نويسنده Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Nikbakht, Zohreh
كليدواژه :
E. coli , Essential oil , Anti-bacterial , Melissa officinalis
چكيده لاتين :
Background: Therapy of plant is not any side effects and drug resustant for inhibition of disease in world. In this study, antimicrobial effects of Melissa officinalis L. ethanol exteract and essential oil on E. coli have been investigated.
Materials and Methods: In this investigation, diameter of inhibitory zone of these materials was measured in disk agar diffusion method. Therefore, Melissa officinalisL. ethanolic exteract (80%) and essential oil have been supplied. Then, antimicrobial activity these substances next for 24 hour for ethanol extraction 80% concentration 50 to 1000 mg/mL and essential oil concentration with 3% to 100% has been considered. Also, comparison of mean diameter of inhibitory zone between treatment and control groups of ANOVA has been used.
Results: Results of these investigations were shown that extract ethanol Melissa officinalis L. was any inhibitory effect on E. coli growth 24 hour after of treatment. Also, diameter of inhibitory zone for 100% essential oil have been 33.2±0.13mm in E. coli that has been increased in comparison control groups significantly (p=0.001). This inhibitory effects was more than cefixime and cefteriaxone (p=0.00, p=0.01).
Conclusion: These found were shown that this plant prevents growth of E. coli In vitro condition. This essential oil could be suggested as antimicrobial agents for inhibition of bacterial diseases in human.
