تنوع ژنتيكي ژنوتيپ هاي محلب (L. Prunus mahaleb) بر اساس صفات مورفولوژيكي و نشانگرهاي RAPD

Genetic Variation of Mahaleb (Prunus mahaleb L.) Genotypes Based on Morphological Traits and RAPD Primers

ندارد

Mahaleb (Prunus mahalab L.) is used as a rootstock for cherries in Iran and many other countries. In this research, RAPD markers were used to determine the genetic diversity among eight genotypes of Mahaleb evaluated in two separate research grardens in Karaj, Iran. These genotypes are used as mother plants for seed production. Totally 40 random primers were examined for PCR reactions on the template DNAs extracted from leaves, of which 12 primers producing polymorphic bands between genotypes were selected. These 12 primers produced 73 bands, among them, 30 were polymorphic. The highest similarity (0.82) was detected between genotypes no. 4 (Gen4) and 7 (Gen7) and the lowest similarity (0.26) between genotypes no. 2 (Gen2) and 5 (Gen5) with genotype no. 8 (Gen8). In another trail, 16 morphological traits were recorded for these genotypes. Resulted morphological cluster, divided these genotypes into three clusters. In plot analysis gnotypes Gen4, Gen1 and Gen2 were separated from others.