مقاومت استافيلوكوكوس اوريوس به روغن كاج (pine oil) با استفاده از تست آنتي بيوگرام و مشاهده باند ژن هاي sigB وmecA در PCR

Staphylococcus aureus resistance to pine oil

Abstract Background and objective: Increasingly use of antibiotics and biocide materials and using various combinations such as pine oil in biocide materials has led to the appearance of pine oil resistant staphylococcus aureus strains. Purpose of this study was to do mutations in S. aureus resistance to pine oil and prove it by testing antibiogram and detect bands of sigB, mecA genes in PCR. Materials and methods: one hundred samples of Staphylococcus aureus from clinical sources were isolated. It was studied its sensitivity and resistance to biocide material of pine oil and two antibiotics effective on cell wall such as oxacilin and vancomycin by phenotypic method. To prove the phenotypic results obtained, presence or absence of resistance genes, mecA and sigB, measured and evaluated by PCR. Results: Within 100 collected samples, 90% were resistant against oxacilin antibiotic and 60% were resistant to biocide material of pine oil and all samples were sensitive to vancomycin antibiotic. Eighty percent of pine oil resistant S.aureus, also were resistant to oxacilin. Conclusion: This research has studied presence of mecA and sigB genes within Staphylococcus aureus for the first time in Iran. mecA gene induct persistence to antibiotics effective on cell wall such as oxacilin and biocide materials such as pine oil. Relation between antibiotic resistance and use of biocide materials, which was proved by presence of this gene, indicates importance of using antibiotics and biocide materials in houses and clinical places.