اثر تركيب عصاره آبي زعفران و تمرين هوازي روي برخي شاخص‌هاي استرس اكسيداتيو كبدي موش‌هاي نر ديابتي

Effect of combination of aerobic exercise and oral administration saffron extract on antioxidant enzymes activity in male rats liver of diabetic by sterpetozotosin

هدف از تحقيق حاضر بررسي اثر تركيب عصاره آبي زعفران و تمرين هوازي بر غلظت آنزيم‌هاي آنتي‌اكسيداني در كبد موش‌هاي صحرايي ديابتي‌شده با استرپتوتوزوسين بود. در اين تحقيق كه از نوع تجربي بود، 70 موش صحرايي ويستار نر به‌صورت تصادفي در پنج گروه شامل كنترل سالم، كنترل ديابتي، ديابتي با تمرين هوازي، ديابتي با مصرف عصاره آبي زعفران، ديابتي با تركيب تمرين هوازي و مصرف عصاره آبي قرار گرفتند. نمونه‌ها دو هفته (14جلسه) به تمرين و مصرف زعفران پرداختند و در پايان فعاليت آنزيم‌هاي آنتي‌اكسيداني سوپراكسيدديسموتاز، گلوتاتيون پراكسيداز و كاتالاز كبدي آنها ارزيابي شد. مقايسه گروه‌ها از لحاظ آماري، با آزمون آناليز يكطرفه (ANOVA) و آزمون تعقيبي شفه انجام گرفت. نتايج نشان داد، تمرين هوازي و مكمل‌سازي زعفران همزمان در سطح معنا‌داري(P < 0/05) سبب كاهش (GPX)، و با همان سطح معنا‌داري(P < 0/05) موجب افزايش(SOD) نسبت به گروه كنترل ديابتي شد. همچنين تركيب تمرين هوازي و مصرف عصاره آبي زعفران به تغيير معنا‌داري در فعاليت آنزيم كاتالاز كبدي منجر نشد.

The aim of present study was effect of combination of aerobic exercise and oral administration saffron extract on antioxidant enzymes activity in male ratsʹ liver of diabetic by sterpetozotosin. In this laboratory experimental research seventy male wistar rats were randomly assigned into five groups including; healthy control , diabetic control, diabetic with aerobic exercise , diabetic with oral administration of saffron extract , diabetic with combination of aerobic exercise and oral administration of saffron extract. The experimental rats were treated in related groups for 2 weeks and at the end of experiment anti-oxidant enzymes activity including superoxide dismutase (SOD), glutathione peroxides (GPX) and catalane (CAT) were measured in liver. Statistically comparison of the groups was carried out using one-way analysis of variance followed by Schaffer post-hoc test. Differences were considered statistically significant at p < 0.05. Results: Results of this study demonstrated that combination of aerobic exercise and oral administration of saffron extract caused significant decrease in the activity of GPX of liver cells; this decrease was the greatest in diabetic control group. Combination of aerobic exercise and oral administration of saffron extract caused significant increase in the activity of SOD of liver cells .this increase was the greatest in diabetic whit aerobic exercise. Also combination of aerobic exercise and oral administration of saffron extract resulted no significant change in the activity of CAT of liver cells in neither of groups. Conclusion: Generally we can say that combination of aerobic exercise and oral administration of saffron extract can uses as one effective treatment method for prevent than frequent complication of diabetic disease