بررسي تاثير عصاره آبي- الكلي هسته زيتون(Olive sativa) بر ميزان هورمون-هاي گنادوتروپين و استروييدهاي تخمداني در موش صحرايي ماده نابالغ

The effects of hydro-alcoholic extract of olive seed on the levels of the gonadotropines and ovarian steroids in immature female rat

مقدمه و هدف:اثرات جانبي داروهاي باروري محققان را بر آن داشته است تا در زمينه تاثير داروهاي گياهي بر فرآيند توليد مثل، بررسي هاي مختلفي را انجام دهند. هدف از اين مطالعه بررسي تاثير عصاره آبي-الكلي هسته زيتون بر ميزان هورمون هاي LH،FSH، استروژن و پروژسترون در جنس ماده بررسي شد. رو ش كار: در اين مطالعه تجربي40 سرموش صحرايي ماده نابالغ از نژاد ويستار با اختلاف وزن كمتر از ده درصد و سن تقريبي 4-5 هفته در گروه هاي10 تايي به شرح زير تقسيم شدند.گروه كنترل: شامل10 سر حيوان كه در زمان آزمايش (28روز) ماده خاصي دريافت نكرد.گروه شاهد: شامل10سرحيوان بود كه يك ميلي ليترآب مقطر به عنوان حلال دارو به مدت28 روز دريافت نمود.گروه هاي تجربي1و2 شامل1 سر حيوان بود كه عصاره هسته زيتون را به ميزانmg/kg250و500 به مدت28روز به روش خوراكي دريافت كردند.از تمام حيوانات در پايان دوره آزمايش نمونه هاي خوني تهيه و ميزان هورمون هايLH،FSH،استروژن و پروژسترون به روش RIA اندازه گيري و نتايج حاصله با استفاده از آزمون هاي آماري آناليز واريانس يك طرفه به همراه آزمون تعقيبي توكي ارزيابي شدند (05/0 > P). يافته ها:غلظت سرمي هورمونLH درگروه هاي تجربي1و2 نسبت به گروه هاي كنترل و شاهد افزايش معني داري را نشان داد.همچنين غلظت سرمي هورمونFSH،استروژن و پروژسترون در گروه تجربي 2افزايش معني داري نسبت به گروه هاي كنترل و شاهد نشان داد. نتيجه گيري:با توجه به نتايج حاصله عصاره آبي-الكلي هسته زيتون باعث تشديد فعاليت محورهورموني هيپوفيز-تخمدان وافزايش ميزان هورمون هاي LH،FSH،استروژن و پروژسترون در موش صحرايي ماده نابالغ مي شود.

Inroduction& Objective: Given the side effects of fertility drugs and other therapies,the researchers have embarked on investigating the effects of medicinal plants on reproductive processes.This study seeks to investigate the effects of hydro-alcoholic extract of olive seed on the levels of LH,FSH,estrogen and progesterone in immature female rat Materials and Methods:The study is of experimental type carried out under laboratory conditions.In doing so,40 immature female Wistar rats,with weight difference of less than10%and age range of4-5weeks were entered into the study.The rats were divided into groups of ten as follows:control consisting of 10rats receiving nothing during the trial period of28day.sham group consisting of10rats which received 1 ml distilled water as drug solvent for 28 days,As for the experimental groups,there were 2 groups of 10 treated as follows:Experimental group consisting of two subgroups of ten receiving orally varying amounts of olive seed extract,250mg/kg and 500mg/kg for a period of 28 days.Blood sampling was performed on all the rats at the end of 28 days from ventricular region of their hearts and levels ofLH,FSH, estrogen,progesterone were measured using RIA method (technique).The obtained data were statistically analyzed by one way Anova and Tukey’s post hoc test.The level of significance was set at p < 0/05. Results: Statistically,a significant increase in LH hormone serum concentration was in the experimental groups receiving varying amounts of hydro alcoholic extract of olive seed after 28,compared to that in the control and sham groups.FSH serum concentration increased significantly in the experimental group receiving 500mg/kg of the extract,after28 days.As for estrogen concentration, the respective level rose significantly in the experimental group receiving 500 mg/kg after 28 days .Also, a significant increase in progesterone level was observed in the experimental group with 500mg/kg extract treatment after 28 days Conclusion:Taking into account the present study findings, we can conclude that hydro-alcoholic extract of olive seed can enhance the ovarian pituitary hormone axis activities, i.e, increase in the levels of the hormones LH, FSH,estrogen,Progesterone in immature female rats. Keywords: Olive seed, LH,FSH,Estrogen, Progesterone