اثرات عصاره ي آبي الكلي گياهان بيلهر زنيان و سنبله كرك دار بر تكثير اليگودندروسيت هاي مغز موش صحرايي

The Effect of Hydroalcoholic Extracts of Dorema aucheri Stachys pilifera and Trachyspermum copticum on Proliferation of Rat Brain Oligodendrocytes

زمينه و هدف: گياهان بيلهر، زنيان و سنبله‌ي كرك?دار در طب سنتي ايران به‌ويژه در كهگلويه و بويراحمد استفاده مي?شوند. هدف اين مطالعه بررسي اثرات اين گياهان بر تكثير سلول?هاي اليگودندروسيت مغز موش صحرايي است. روش بررسي: با استفاده از اتانول 50 درصد از گياهان مذكور عصاره‌ي آبي الكلي خيسانده تهيه شد. سلول‌هاي رده‌ي اليگودندروسيت مغز (OLN-93) براي 24 ساعت با غلظت‌هاي مختلف اين عصاره‌ها تيمار شدند. سپس درصد سلول‌هاي زنده با روش احياي نمك تترازوليوم ارزيابي شد. يافته‌ها: عصاره‌ي بيلهر در غلظت‌هاي 50، 100، 200 و 400 ميكروگرم در ميلي ليتر درصد سلول‌هاي زنده را از 4±100 (سلول‌هاي شاهد) به‌ترتيب به 4±84، 6±58 (001/0P < )، 5/0±6 (001/0P < ) و 5/0±4 درصد (001/0P < ) كاهش داد. عصاره‌ي سنبله كرك‌دار نيز در غلظت‌هاي 50، 100، 200 و 400 ميكروگرم در ميلي?ليتر باعث كاهش سلول‌هاي زنده به?ترتيب از 4±100 به 6±81، 3±64 (01/0P < )، 4±61 (01/0P < ) و 2±55 درصد (001/0P < ) شد. همچنين عصاره زنيان در غلظت‌هاي بيشتر از 50 ميكروگرم در ميلي?ليتر درصد سلول‌هاي زنده را به طور معني?داري كاهش داد. نتيجه گيري: عصاره‌ي بيلهر، زنيان و سنبله‌ي كرك‌دار تكثير سلول‌هاي اليگودندروسيت مغز موش صحرايي را كاهش مي‌دهند. واژگان كليدي: بيلهر، زنيان، سنبله كرك دار، اليگودندروسيت، موش صحرايي

Background and Objective: Dorema aucheri, Stachys pilifera and Trachyspermum copticum are used in Iranian traditional medicine especially in the province of Kohgilouyeh and Boyerahmad. This study aimed to determine the effect of these plants on proliferation of rat brain oligodendrocytes. Materials and Methods: Macerated hydroalcoholic extract of each plant was prepared using 50% ethanol. The OLN-93 oligodendrocytes were incubated for 24 h with different concentrations of these extracts. Then, percentage of viable cells was determined using a method based on the reduction of tetrazolium salt. Results: Extract of Dorema aucheri at 50, 100, 200, and 400 µg/ml decreased viable cells from 100 ± 4% (control cells) to 84 ± 4, 58 ± 6 (P < 0.001), 6 ± 0.5 (P < 0.001) and 4 ± 0.5% (P < 0.001), respectively. Similarly, Stachys pilifera extract at 50, 100, 200 and 400 µg/ml decreased viable cells from 100 ± 4% to 81 ± 6, 64 ± 3 (P < 0.01), 61 ± 4 (P < 0.01) and 55 ± 2% (P < 0.001), respectively. Also, Trachyspermum copticum extract at concentrations higher than 50 µg/ml significantly decreased percentage of viable cells. Conclusion: Extracts of Dorema aucheri, Trachyspermum copticum and Stachys pilifera reduce proliferation of rat brain oligodendrocytes. Keywords: Background and Objective: Dorema aucheri, Stachys pilifera and Trachyspermum copticum are used in Iranian traditional medicine especially in the province of Kohgilouyeh and Boyerahmad. This study aimed to determine the effect of these plants on proliferation of rat brain oligodendrocytes. Materials and Methods: Macerated hydroalcoholic extract of each plant was prepared using 50% ethanol. The OLN-93 oligodendrocytes were incubated for 24 h with different concentrations of these extracts. Then, percentage of viable cells was determined using a method based on the reduction of tetrazolium salt. Results: Extract of Dorema aucheri at 50, 100, 200, and 400 µg/ml decreased viable cells from 100 ± 4% (control cells) to 84 ± 4, 58 ± 6 (P < 0.001), 6 ± 0.5 (P < 0.001) and 4 ± 0.5% (P < 0.001), respectively. Similarly, Stachys pilifera extract at 50, 100, 200 and 400 µg/ml decreased viable cells from 100 ± 4% to 81 ± 6, 64 ± 3 (P < 0.01), 61 ± 4 (P < 0.01) and 55 ± 2% (P < 0.001), respectively. Also, Trachyspermum copticum extract at concentrations higher than 50 µg/ml significantly decreased percentage of viable cells. Conclusion: Extracts of Dorema aucheri, Trachyspermum copticum and Stachys pilifera reduce proliferation of rat brain oligodendrocytes. Keywords: Dorema aucheri boiss, Stachys pilifera, Trachyspermum copticum, Oln-93 cell line, Antiproliferative effect , Stachys pilifera, Trachyspermum copticum, Oln-93 cell line, Antiproliferative effect