بررسي تنوع زيستي باكتريهاي نمك دوست نسبي و تحمل كننده نمك قابل كشت در تالاب پرشور اينچه برون

Diversity of culturable moderate halophilic and halotolerant bacteria in incheh boroun hyper saline wetland in Iran

تالاب اينچه برون در شمال ايران در نزديكي مرز تركمنستان واقع شده و شوري و تغييرات pH آن قابل توجه است . در شهريور 1389 از آب ، خاك و نمك 4 منطقه تالاب نمونه برداري شد. 400 جدايه خالص سازي و 55 سويه PCR گرديد. جنسهاي Bacillus (18 درصد)، Marinobacte (16 درصد)،Halomonas (16 درصد)، Kocuria (9 درصد)،Oceanobacillus (7 درصد)،Dietzia (7 درصد)،Virgibacillus (6 درصد)،Chromohalobacter (5 درصد)،Rhodococcus (2 درصد) ، Micrococcus (3 درصد)، Paenibacillus (2 درصد)، Halobacillus (3 درصد)، Thalassobacillus (2 درصد)، Arthrobacter (2 درصد) وDesmospora (2 درصد) جدا شدند. در 13 سويه ميزان شباهت در ترادف ژن 16S rRNA بين 4/98-97 درصد ودر 7 سويه كمتر از 97 درصد بود كه بيانگر تفاوت قابل ملاحظه اي در سطح گونه و يا در برخي حتي جنس است . با بررسي بهينه رشد نمكي 22 سويه نمك دوست نسبي و33 سويه تحمل كننده نمك بودند. توليد 4 آنزيم هيدروليتيك بررسي گرديد كه عمده ترين توليد كنندگان آن باسيلهاي گرم مثبت و بيشترين فراواني متعلق به آنزيمهاي ژلاتيناز و پروتياز بود .

Incheh Borun hypersaline wetland is located near the border of Turkmenistan,in north of Iran. This wetland is remarkable because of salinity and variation in pH range. sampling was carried out from soil, water and salt in September 2010. 400 strains were purified and 55 strains were selected randomly for PCR. Genera: Bacillus (18%), Marinobacter (16%), Halomonas (16%), Kocuria (9%), Oceanobacillus (7%), Dietzia (7%), Virgibacillus (6%), Chromohalobacter (5%), Rhodococcus (2%), Micrococcus (3%), Paenibacillus (2%), Halobacillus (3%), Thalassobacillus (2%), Arthrobacter (2%) and Desmospora (2%) were isolated 13 strains showing 97-98.4 % similarity and 7 strains had less than 97% similarity in 16S rRNA sequencing which is showed significant differences in the level of species or even genus. Optimum growth for salt was evaluated and 22 strains were moderate halophile and 33 strains were halotolerant. Producing 4 hydrolytic enzymes was evaluated which the main producers of hydrolytic enzymes were Gram-positive bacilli and the most frequent enzyme was Gelatinase and Protease.