تعيين غلظت كشنده نانو نقره در ماهي گورخري معمولي (Rüppell, 1829) Aphanius dispar

Effect of lethal concentration of Nano Silver in Aphanius dispar

هدف از پژوهش حاضر، مطالعه سميت حاد كلوييد نانو ذرات نقره از طريق تعيين غلظت كشنده مياني (LC50) آن در ماهي گورخري معمولي Aphanius disparبود. آزمايشات سم‌‌شناسي طبق استاندارد شماره 203 OECD، طي 96 ساعت انجام و تلفات ماهيان پس از رويارويي با غلظت هاي اسمي 0، 5، 10، 20، 30، 40 و 50 ميلي گرم در ليتر نانو ذرات نقره، هر 24 ساعت يكبار ثبت شد. داده‌هاي حاصل از مرگ و مير ماهيان به كمك نرم افزار probit آناليز گرديد. مقدار غلظت كشنده مياني 96 ساعته كلوييد مذكور، 631/10 ميلي گرم در ليتر به دست آمد. بنابراين طبق سيستم هماهنگ جهاني (GHS) طبقه بندي و برچسب گذاري مواد شيميايي، نانو نقره مورد مطالعه براي ماهي مذكور جزو گروه 3 حاد طبقه بندي شده و بر اساس قوانين استاندارد اتحاديه اروپا، حضور اين ماده در محيط زيست اين ماهي مضر مي‌باشد.

Rising trend of production and use of nanoparticles can lead to the possibility of their release into aquatic environments. Therefore, knowing toxic effects of these materials on aquatic organisms is of great importance. The objective of this research was to study the acute toxicity of colloidal silver nanoparticles (CAgNPs) through determining the lethal concentrations in the euryhaline teleost, Aphanius dispar. Toxicology experiments were conducted for 96 h in freshwater according to the OCED 203 standard protocol. Fish mortality was recorded every 24 h after exposure to concentrations of CAgNPs 0, 5, 10, 20, 30, 40 and 50. The actual concentration of silver in each aquarium was measured by atomic absorption and based on the actual concentration; data on mortality were analyzed using the US EPA Probit analysis program. Based on the results, the estimated 96h LC50 of CAgNPs was 10.631 mg/L. Accordingly, the tested CAgNPs should be classified according to GHS (Globally Harmonized System of Classification and Labeling of Chemicals) as “category acute 3” for Aphanius dispar; this means that presence of CAgNPs in the environment of this fish is detrimental and, therefore, its release into the aquatic ecosystem should be carefully considered.