سيستم فيبرينوليز را مهار مي كنند MC2B2 و A2C8 آنتي باديهاي منوكلونال پلاسمينوژن انساني

A2C8 and MC2B8 Monoclonal Antibodies Against Human Plasminogen inhibit fibrinolytic System

In the present study the effects or two antiplasminogen monoclonal antibodies A2C8 and MC2B8 on the activation of the fibrinolytic system was studied. For this purpose, after separate steps like the culture of antibody producing hybridoma cells, their injection to mice, and purification of antibodies from ascites fluid, the effects of these antibodies was studied with various methods. Primary observation with pooled plasma showed that these antibodies in the presence of plasminogen activators (t-PA, u-PA and SK) can inhibit the activation of fibrinolytic system. According the results obtained from D-dinner assay. the inhibition effects of these antibodies is dose dependent. The other test that performed with S-2251 synthetic substrate showed that the activation of plasminogen in the presence of Urokinase and in the result, deterioration of this substrate was decreased in the presence of A2C8 and MC2B8 antibodies. In the present study we evaluated the effects and mechanism of two antiplasminogen monoclonal antibodies A2C8 and MC2B8 on the activation of the fibrinolytic system in the presence of plasminogen activators.