به قشر انتورينال بر تشنج هاي ناشي از كيندليگ الكتريكي قشر پيريفورم در موش صحرايي A2A تاثير تزريق آگونيست اختصاصي گيرنده هاي آدنوزيني

The effect of intraentorhinal injection of selective A2A receptor agonist on piriform cortex-kindled seizures in rats

The role of adenosine A2A receptors within the entorhinal cortex on piriform cortex- kindled seizures was investigated in this study. Animals were kindled by daily electrical stimulation of piriform cortex and CGS21680 hydrochloride (CGS), an adenosine A2A receptor agonist at concentrations of 500 .tM and 1 mM were bilaterally injected into the entorhinal cortex. The results showed that CGS at a concentration of 1 mM, 5 min after injection, increases the after-discharge duration (ADD), and has no effect on the latency up to stage 4 (S4L), stage 5 seizure duration (S5D), and seizure duration (SD), and no alteration was observed at a concentration of 500 W. In addition, bilateral microinjection of ZM241385 (ZM), an adenosine A2A receptor antagonist, into the entorhinal cortex at concentrations of 200 and 500 1.tM had no effect on seizure parameters. Meanwhile, pretreatment of animals with ZM (200 µM) 5 min before CGS (1 mM) eliminated the excitatory effect of CGS on seizure parameters. These results suggest that stimulation of the adenosine A2A receptors within entorhinal cortex enhances the intensity of piriform cortex- kindled seizures.