خصوصيات ريولوژيكي عصاره شيرين‌بيان

Rheological properties of Liquorice (Glycyrrhiza glabra) extract

شيرين‌بيان يكي از مهمترين گياهان دارويي و صنعتي است كه در بسياري از صنايع كاربرد دارد. ريشه شيرين‌بيان تركيبات متنوعي دارد. عصاره آبي ريشه شيرين‌بيان بدليل خواص فيزيكي عملكرديش كاربردهاي متنوعي در دارو سازي و صنايع ‌غذايي پيدا كرده است. رفتار ريولوژيكي عصاره شيرين‌بيان و مطالعه اثر درجه حرارت و مواد جامد محلول بر ويسكوزيته عصاره در محدوده دمايي 70-10 درجه سانتي‌گراد و غلظت 50-5 درصد با استفاده از دستگاه ويسكومتر بروكفيلد بررسي شد. نتايج، رابطه خطي تنش برشي-سرعت برشي را در غلظت‌هاي مختلف و دماهاي مورد مطالعه نشان داد كه بيانگر نيوتني بودن سيال بود. همچنين كاهش ويسكوزيته در اثر افزايش دما در نمونه‌ها مشاهده شد و همچنين افزايش درصد غلظت نمونه‌ها موجب افزايش ويسكوزيته شد.

Introduction: Liquorice (Glycyrrhiza glabra) of the family Leguminosae is a 30±60 cm tall shrub having blue or violet flowers. Its roots are 15±20 cm long and have a cylindrical rod shape of 0.5±2.5 cm in diameter. Liquorice root contains a variety of compounds, the most prominent of which is the water soluble triterpenoid glycoside glycyrrhizin that constitutes about 16 %. It is responsible for the characteristic sweet taste of liquorice (50 times sweeter than sucrose). The root also contains various sugars (to 18%), flavonoids, saponoids, sterols, starches, amino acids, gums, and essential oil. The water extract of liquorice root has found wide uses in medicine, pharmacology and food industry because of its physical and functional properties. In the food industry, its distinctive flavour makes it a popular sweetening/flavouring agent. It is also used as an emulsifier to create foam in drinks and beverages. Liquorice root extract is commonly consumed and marketed in liquid, concentrated, dry and powdered forms. Knowledge of the flow (rheological) behavior of the concentrates is useful in quality control, sensory evaluation and engineering applications that are related to the proper design and operation of unit operations, as well as for the understanding of the pertinent transport processes in the operations. The purpose of this investigation was to characterize the rheological behavior of liquorice extract concentrates and study the effect of temperature and soluble solids on the viscosity. Materials and methods: Extraction was made at 50°C and water to liquorice root mass ratio of 4:1. The mixture was shaken continuously for 100 min, centrifuged for 10 min and then filtered to obtain a clarified extract, which was concentrated to 50°Brix in a laboratory rotary vacuum evaporator. The rheological behavior of licorice extract has been studied at temperature range 10-70 0C and 5-50 % soluble solids using Brookfield viscometer. Results and discussion: The obtained results showed that all the samples exhibited Newtonian behavior at all the studied temperatures and concentrations. The flow index decreased slightly with sample concentration. Several workers observed Newtonian behavior of clarified and depectinated juices. The shear stress versus shear rate data obtained were well fitted to Newtonʹs model. An increase in temperature decreased the viscosity and this inverse relationship has been likened to the incidence of a freer molecule to molecule interaction at elevated temperatures. Since viscosity is an indication of the resistance to flow such a freer interaction is expected to minimize the resistance. It was observed that, irrespective of the temperature a decrease in soluble solids content decreased the viscosity. It is due to dilution which increases the separation between the particles, thus decreases interaction between the particles. In contrast, the opposite effect was observed when soluble solids content became higher. Arrhenius type equation was found to be suitable in describing the relationship with correlation coefficient values between 0.94 and 0.99. The activation energy increased with the soluble solids content; therefore, temperature had a greater effect on samples with higher soluble solids content. The data obtained in this study can be used in designing equipment for handling of concentrated liquorice root extract at the temperature range studied.