پديد آورندگان :
حيدرزادي، كبري نويسنده كميته تحقيقات دانشجويي, دانشگاه علوم پزشكي ايلام,ايلام,ايران Heidarzadi, Kobra , عزيزي جليليان، فريد نويسنده دانشكده پزشكي,گروه ميكروب شناسي, دانشگاه علوم پزشكي همدان,همدان,ايران , , ركابي، عليرضا نويسنده دانشكده دندان پزشكي,گروه پريو,دانشگاه علوم پزشكي ايلام,ايلام,ايران Rekabi, Ali Reza , اميني، راضيه نويسنده دانشكده پزشكي,گروه پزشكي مولكولي,دانشگاه علوم پزشكي همدان,ايران , , پاكزاد، ايرج نويسنده دانشكده پزشكي,گروه ميكروب شناسي, دانشگاه علوم پزشكي ايلام,ايران Pakzad, I. , طاهري كلاني، مروت نويسنده دانشكده پزشكي, گروه ميكروب شناسي,دانشگاه علوم پزشكي ايلام,ايران Taherikalani, Morovat , همتيان، علي نويسنده دانشكده پزشكي,گروه ميكروب شناسي,دانشگاه علوم پزشكي ايلام,ايران Hematian, Ali , قباديان، زهرا نويسنده كميته تحقيقات دانشجويي,دانشگاه علوم پزشكي ايلام,ايران , , بوچاني، مريم نويسنده كميته تحقيقات دانشجويي, دانشگاه علوم پزشكي ايلام,ايران Bouchani, Maryam
چكيده لاتين :
The most common method of oral hygiene is tooth brushing. However, occasionally brushing becomes a risk factor for health. The main reason is getting infected with various microorganisms. The purpose of this study was to investigate bacterial and fungal contamination of toothbrushes students, and factors affecting the contamination.
Materials Methods: Ninety five brushes were assessed in this study. Sles were incubated in test tubes containing nutrient broth for twentyfour hours. Then the microorganisms were isolated using specific nutrient medium containing Blood agar, Chocolate agar MacConkey and sabouraud dextrose agar. The inoculated plates were incubated for 24 to 48 hours at 37 C. The identification of microorganisms was performed based on biochemical tests.
Findings: The infection rate was more than 40% of the subject staphylococcus, Niesseria, Diphteroids,Actinomycetes and Enterobacteriacae family members were isolated from sles. Microbial contamination was higher in women than men (P<0.05).There was found correlation between the number of microorganism on brush with time of use, using mouthwash and gender (P<0.05). There was statistically significant relation between cocci microorganismsrsquo infections with sexes as well as between bacillus infections with capped brush.
Discussion Conclusion: Based on results, high incidence of bacterial contamination observed in brushes, therefore the proper use, maintenance and timely replacement of toothbrush play an important role in reducing pollution and consequently decreasing oral disease.