بررسي اثر تزريق داخل صفاقي خردل گوگردي (سولفور موستارد) بر سلولهاي جامي ژژنوم موش بزرگ آزمايشگاهي

Effects of Sulfur Mustard on the Jejunal Goblet cells in NMRI Rats

Sulfur mustard has not any excitatory or primary sensory effects one hour after use. For this reason it was used during the imposed war by IRAQ against IR.IRAN. Necrosis of the epithelial cells in stomach and intestines, Severe diarrhea, reduction and inhibition of cell division, weight loss and death are the common clinical problems of this chemical agent. Attention to the importance of jejuna] absorption of nutrients and the role of goblet cells has been assessed to study the acute and chronic effects of sulfur mustard on these cells in this study 78 NMRI male mature rats weighting 200 40 gram were selected and divided into 13 groups. One group received Thyrodʹs baffer as a solvent of SM sham group). The other group as control group did not receive any injection. In the other 9 groups different doses of SM (2.5, 5, 10 mg/kg) were injected after 24 hours , 2 weeks and 8 weeks. In last 2 groups different doses 20,40 mg/kg were used and followed for 24 hours of SM. Samples were taken from first 1 cm of jejunum and fixed in Bowins solution, dehydrated and then embeded in parafin wax and serially sectioned (UM). Sections were stained by Hematoxyline/ Eosin. Microscopic eye piece (Holland eye piece HWF 1 OX) were used for cell counting. The result indicated that SM has direct effects. on gastrointestinal tract and disturbance of digestion and absorption. It reduces appetite weight loss was significantly more compared to sham and control groups. But this effect was reduced by the time. SM also causes reduction in the number of jejunum goblet cells. which is related to the doses of SM. According to the result, this reduction was signifient (P<0.001) in group 24 hours with 20 , 40 mg/kg dose and 2 weeks with 5,10 mg/kg dose,and eight weeks with all three doses(2.5, 5, 10mg/kg).