كليدواژه :
عاج ثانويه , ژلاتين ماتريكس استخواني داخل غضروفي , تمايز سلولهاي پالپي , Endochondral Bone Matrix Gelatin , Secondary Dentin , Pulp Cell Differentiation
چكيده لاتين :
The Endochondral Bone Matrix Gelatin (Ec BMG) and its derivative materials are used for bone induction. However we used of Ec BMG in the pulp cavity for cell differentiation and secondary dentin formation. This research designed to evaluate the pulp cell (Ectomesenchymal cells) differentiation and secondary dentin formation by using Ec BMG in Deutch-Poland rabbits. Ec BMG was prepared from long bones (femur, tibia, humerus, radius and ulna) of rabbits ( weight 1.5 kg) as previously described by Urist. Twelve male rabbits were divided in to three groups. In each group, the right mandibular incisive tooth was implanted with BMG as the experimental groups. The left sides were used as control groups. The animals were killed at 7, 14 and 21 days after operation. The teeth were extracted and processed by routine histological procedures, stained (by Hematoxilin and Eosin and Trichrom Masson) and studied by ligth microscope. Seven days after operation the differentiation of pulp cells and secondary dentin formation were not detectable and only inflammatory cells appeared around the BMG particles. At day 14th after operation, the BMG particles were surrounded by odontoblast like cells and seconday dentin was seen. Twenty- one days after operation, odontoblast like cells and secondary dentin appeared in the pulp cavity and BMG particles were absorbed almost entirely.
In conclusion, the Ec BMG particles have the capacity to differentiate pulp cells to odontoblast like cells and form secondary dentin. Therefore, it seems, the BMG may have clinical use for dentinogenesis.
