بررسي اثرات نيتروفورازون و نيتروفورانتويين بر اسپرماتوژنز در موشهاي صحرايي نر بالغ

Nitrofurazone and Nitrofurantoin Antifertility Effect Evaluation on Adult Male Rats

Many studies are done to show different drugs and compounds with contraceptive activity in male. In this study, we have examined the effect of nitrofurantoin and nitrofurazone on testicular tissue and spermatogenesis in adult male rats, using histologic and histomorphometric methods. Test compounds were injected subcutaneously. Male Sprague-Dawley rats, in 15 mg/kg doses twice per day for 50 days. The rats were euthanized by decapitation, and thier testis were immediately fixed in 10% formalin. After subsequent preparation, the sections with 5 micrometer thickness were prepared and stained by the H and E technique. By using software Live 3000 our histomeric studies were computed. The results were as follow: The diameter of seminiferous tubules reduced and the results were consistent with the reduction of circumference and areas of these tubules and subsequent increase in the intratubular space and distance. Atrophy and deformation of seminiferous tubules were seen, in the test groups. Increase in the number of Leydig cells, and reduction of nuclear volume were observed in Nitrofurazone group, but no changes were observed in nitrofurantoin group. Narrowing of tubules and thier blockade by eosinophilic masses were observed in the both experimental groups. Decrease in the number of the spermatogonium class cells within seminiferous were observed in the test groups and spermatogenesis up to the zygoten cells stage were inhibited due to the adminstration of the test compounds. Our finding show that Nitrofurantoin reduced the formation of spermatozoids but did not have adverse effect on Leydig and Sertoli cells, and these cells are remained nearly intact at the end of the experiment and therefore can be considered in the search for the development of a new male contraceptive agent.