A اثرات سولفات مس بر تندش اسپور رشد و توليد توكسين از كلستريديوم برتولينوم ثيپ

The Effects Of Copper Sulfate On Spore Germination, Outgrowth and Toxin Production Of Clostridium Botulinum Type A

The principal hazard of clostridia in humans is the lethal toxin production. In order to prevent food poisoning and botulism infection, the search for new methods to control bacterial growth, toxin production or its inactivation is necessary. In this investigation, the effect of copper sulfate, and incubation time on germination time of spore, growth and toxin production of clostridium botulium type A were studied. Samples with different concentrations of copper sulfate were inoculated with I.06 cfu/ml spores, or with 107 cfu/ml bacterial suspension, anearobically incubated at 35°C, and thenafter analyzed for spore germination, outgrowth and toxin production. The results show that copper sulfate at 5 to 40 ,ug/ml concentration effectively increase germination and outgrowth of clostridium botulinum type A. and a protein which reacted with specific anti-botulinum toxin type A was produced; however, no toxicity was detected in presence of different concentrations of copper sulfate tubes. While elaboration of toxin occurred in control tubes, we found by measurement the amount of copper ion in samples that copper ions have been chilated with toxin and inactivated it.