907882

Characterization of digestive α and βglucosidases in the midgut of Plagiodera versicolora Laicharting (Coleoptera: Chrysomelidae)

Shabarari، M. نويسنده University of Mohaghegh,Iran Shabarari, M. , Naseri، B. نويسنده University of Mohaghegh Ardabili,Iran Naseri, B.

فصلنامه سال 1394 شماره 63

علمي پژوهشي

7

45

51

?-glucosidase , midgut , Plagiodera versicolora , Iran , ?-glucosidase

The imported willow leaf beetle, Plagiodera versicolora is one of the main pests of forest trees worldwide including Iran. Activities of α and βglucosidases in the midgut of P. versicolora adults were studied in this research. The results showed a higher α glucosidase activity of adultsthan βglucosidase. The optimal pHs for α and βglucosidases activities were found to be 6 and 9, respectively. Optimal temperatures for the enzymatic activities were recorded at 30 and 25 °C, respectively. Concentration of 0.5 mM K+ significantly minimized the activity of α glucosidase and maximized the activity of αglucosidase in presence of Ca2+. The activity of βglucosidase was decreased by adding Mg2+ and Zn2+. 5 mM concentration of K+ had the most inhibitory impact on βglucosidase activity, while 0.5 mM concentration of Na+ showed the most positive effect on the enzyme activity. The Km of midgut α and β glucosidase was 0.36 and 2.03 mM and the Vmax value was 5.46 and 18.51 μmol/min/mg protein, respectively.

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نامه انجمن حشره شناسي ايران

نامه انجمن حشره شناسي ايران

فصلنامه با شماره پیاپی 63 سال 1394

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