تاثيري بر سلول كشي ماستوسيتهاي پرورش يافته در مجاورت اينترلوكين -3 ندارد TNF-a فاكتور نكروزدهنده تومور

Natural cytotoxicity (NC) of IL-3 induced mast cell line is not mediated by tumor necrosis factor-alpha (TNF-Alpha)

Long-term culture of CBA mouse spleen cells in 1L-3 conditioned medium induced a stable mast cell line (MCL). This mast cell line expressed NC-L1 receptor that mediates natural cytotoxicity against tumor, but had no markers of T cell, B cells, macrophages, or NK cells. Since in most literature tumor necrosis factor alpha (TN F-a) has been proposed as a major mediator in natural cytotoxicity, and MCL cells has no receptor for TNF-a, in this study we aimed to identify the role of TNF-a in killing of WEHI-164 fibro sarcoma by MCL. MCL and spleen cells from mice (CBA*C57BL/6)FI (Control group) were separately incubated with anti-TNF-a antibody to block the NC. Furthermore to investigate the presence of TNF-a gene in MCL, mRNA of TNF-a gene was studied using RT-PCR experiment. Results showed that natural cytotoxicity of MCL was not blocked by anti-TNF-a compared to that of F1 mice spleen cells (CBA*C57BL/6)F1. Further RT-PCR results showed no constitutive expression of TNF-a mRNA in MCL and upon interaction with WEHI-164 targets, only transient induction of TNF- a mRNA was observed at 30 minutes. We conclude that the NC activity of MCL is mediated by factor(s) other than TNF-Alpha.