بررسي اثر هيپرترمي بعد از تابش نوترون بر فراواني آسيبهاي كروموزومي در لنفوسيتهاي خون محيطي انسان

Effect of hyperthermia after neutron irradiation on chromosomal aberration frequency in human peripheral blood lymphocytes

Nowadays, researchers work on combination therapy for cancer treatment. This study was performed to find the effect of hyperthermia after neutron irradiation on chromosomal aberration frequency in human peripheral blood lymphocytes. Blood samples were irradiated with 10cGy neutron, then they exposed to hyperthermia at 41.5 °C for 60 minutes or at 43°C for 30 minutes with a sixty minute time interval. After tissue culture and preparation of slides, chromosomal aberrations were scored during metaphase. The results showed that post-hyperthermia at both 41.5 and 43 °C increased chromosomal aberration frequency induced by neutron irradiation and it was more remarkable at 43°C (P<0.05). The cause of these effect probably is the inhibitory effect of hyperthermia on repair process of chromosomal damages.