كارآيي جاذب تركيبي مكمل شده با جيره غذايي در كاهش سميت آفلاتوكسين ب1 و بهبود ايمني ماهي قزل آلاي رنگين كمان (Oncorhynchus mykiss)

Capability of dietary supplementation of composite toxin binder in reducing aflatoxin B1 toxicity and its immunomodulation in rainbow trout (Oncorhynchus mykiss) fingerlings

مطالعه حاضر با هدف ارزیابی اثر غلظت‌های مختلف سم آفلاتوكسین ب1 و كارایی نوعی جاذب تركیبی بر شاخص‌های خونی و بیوشیمیایی سرم خون و بافت كبد ماهی قزل‌آلای رنگین‌كمان انجام پذیرفت. به این منظور تعداد 414 قطعه بچه ماهی قزل‌آلای رنگین‌كمان (20/1±67/9 گرم) در قالب 6 تیمار با جیره‌های حاوی 3 سطح آفلاتوكسین (ppb 0، 25 و 50) و دو سطح جاذب (صفر و 5 درصد) به مدت 60 روز پرورش داده شدند. در پایان آزمایش از نظر شاخص‌های خونی، اختلاف معنی داری بین تیمارهای مختلف مشاهده نشد (05/0

In the present study, the effects of aflatoxin B1 (AFB1) and a compound binder on blood cell count, biochemical indices of serum and liver histology of rainbow trout was investigated. In this regard, total number of 414 trout fingerlings (9.67±1.20g) were randomly allocated into six different treatments comprising various dietary inclusion levels of AFB1 (0, 25 and 50 ppb) and binder (0 and 5%) for 60 days. At the end of experiment, blood indices did not differ amongst treatments. The highest and lowest levels of ALP activities were observed in treatments fed diets containing 5% binder-25 ppb AFB1 and the group without any dietary aflatoxin and binder inclusions, respectively (P<0.05). Activities of AST and ALT were only affected by the binder to the extent that its presence led to lower enzymes activity (P<0.05). Interaction of AFB1 and binder significantly affected total protein and globulin content (P<0.05). The albumin content was only affected by the binder (P<0.05). According to the results AFB1 reduced HSI (P<0.05). Blood vessels dilation, cytoplasmic degeneration, blood congestion and hepatic immune cells infiltration were observed in fish fed aflatoxin-contaminated diets which were severe in 50 ppb AFB1-recieved groups. In conclusion, The AFB1 resulted in liver tissue damage and despite improving the immune indices; binder did not show remarkable hepato-protective effects.