RAPD مقايسه شباهت و فاصله ژنتيكي تاسماهي ايراني و تاسماهي روسي با استفاده از روش

Genetic difference and resemblance between Acipenser persicus and Acipenser gueldenstaedtii by means of RAPD Technique

We studied genetic difference and resemblance between Acipenser persicus and Acipenser guldenstaedtii using Random Amplified Polymorphic DNA (RAPD) technique. The DNA of tail fin tissue of three A. persicus and A. gueldenstaedtii were extracted using phenol-chloroform method. After electrophoresis of the samples by agarose gel, their concentrations were regulated and Polymerase Chain Reaction (PCR) was conducted by 53 primers. PCR products were electrophoresed on polyacrylamide gel and silver staining was done to reveal the DNA bands of the samples. Among 53 primers, 17 had no site on genomic DNA of A. persicus and A. gueldenstaedtii and did not produce any bands while the remaining 36 primers showed band pattern. Analyzing the PCR products data using RAPD PLOT program showed that the maximum and minimum genetic distance between species were 73% and 65% respectively. Also, the mean difference between the species was 70% and the maximum and minimum genetic resemblance between the two species were 35% and 27% respectively. Based on the results, we conclude that A. persicus is an independent species from A. gueldenstaedtii.