بررسي تنوع ژنتيكي برنج هاي ايراني با استفاده از نشانگر رپيد

Evaluation of Genetic Diversity of Iranian Rice (Oryza sativa L.) Using RAPD Markers

Rice is an important crop and is cultivated in diverse geographical areas of Iran. The presence of genetic diversity is essential in breeding programs. We have used 113 Iranian rice accessions obtained from the Iranian National Gene Bank Collected from various geographical origins. A set of 14 oligonucleotide primers was used for RAPD analysis. A total 135 reproducibly scorahle RAPD markers were generated across all varieties. Scoring was carried out on the basis of presence (l) and absence ((1) of a given hand. This data was used to construct a resemblance matrix according to Jaccard coefficient. NTSYS program was used to perform cluster analysis and build the dendrogram according to the UPGMA clustering method. These accessions were grouped in seven clusters and similarity among them was 45 - 88%. "Fajr" and "Sahel" with an 80% similarity were the closest improved cultivars. This germplasm showed vast diversity even for accessions with the same names. They are desirable sources for future breeding purposes.