تجزيه كمي و كيفي ساپونين هاي وارتيه هاي مختلف يونجه و بررسي رابطه آن ها با مقاومت به سرخرطومي برگ يونجه

Quantitative and Qualitative Analysis of Saponins in Different Varieties of Alfalfa and their Relationships with Alfalfa Weevil Resistance

Twenty two Iranian and foreign varieties of alfalfa (Medicago sativa L.) were studied with respect to total amount of saponins (quantitative analysis) and kinds of saponins (qualitative analysis). Quantitative analysis was conducted by spectrophotometric method. Varieties showed high significant differences for total amount of saponins. Total amount of saponins showed no significant correlation with alfalfa weevil (Hypera postica Gyll.) larval damage. Qualitative analysis was conducted by TLC method. Eight types of saponins were recognized in the varieties. The comaprison of saponin banding patterns with star lard patterns showed that bands 3,4, and 7 (saponins) were Medicoside J, Soyasaponin 1 and Medicoside A, respectively. Other five saponins could not be recognized with available standard saponins. The 1st, 2nd, and 8th Saponin bands showed significant negative correlations with weevil larval damage, 3rd and 4th Saponin showed significant positive correlations, and 5th, 6th and 7th Saponin bands showed no correlation with larval damage.