PCR- RFLP بخش شمالي (رودخانه ولگا) و جنوبي (رودخانه تجن ) درياي خزر به روش ملكولي ACIPENSER STELLATUS PALLAS, 1771 بررسي ملكولي جمعيت ماهي اوزن برون

Study of mtDNA variation of stellate sturgeon (Acipenser stellatus)population from the north (Volga River) and South (Sefidrud River)Caspian Sea Using RELP analysis of PCR Amplified ND 5/6 gene regions

Genetic variation of Stellate Sturgeon (Acipenser Stellatus) from the North (Volga river) and South (Sefidrud river) Caspian Sea was investigated using NADH 5/6 gene and PCR-RFLP analysis. In this Study a total of 60 samples of Stellate Sturgeon from the North (Volga river) and 13 samples from the Southern (Sefidrud river) Caspian sea were collected. MtDNA ND 5/6 gene was amplified by polymerase chain reaction (PCR). PCR-product digested using 31 endonucleases restriction enzyme. Five enzymes of 31 enzymes showed polymorphism (Rsal, Hinfl, Hpall, Haelll and Mbol). Totally 22 composite haplotypes among 73 samples were detected. Haplotypes AAAAA, BAAAA, ABABA from the Volga river showed 28.33% and 11.66% frequency, respectively, and from the Tajan river haplotypes AAAAA, ABBAA showed 38.46% and 15.40% frequency, respectively. Average haplotype and nucleotide diversity within populations in Volga river was 0.88±0.027 and 0.0122 and in Tajan river was 0.86±0.089 and 0.0093, respectively. Average nucleotide diversity and nucleotide divergence among populations of North and South Caspian Sea were 1.126 and 0.047, respectively. Monte-Carlo simulation using 1000 replication was shown nonsignificant difference between sampling site in the southern and northern Caspian Sea (X^2=0.036±0.006).