به تنش شوري GLYCINE MAX L ارزيابي تحمل كالوس هاي پرتوتابي شده جنين سويا

The study of salt stress tolerance of irradiated calli in soybean(Glycine max. L)

Induction and selection for salt stress tolerance in plants by synchronized application of tissue culture and mutation is a most useful approach for creation of salt stress tolerant plant. In order to study the effect salt stress tolerance of irradiated calli in soybean, an experiment was conducted in 2001 in tissue culture laboratory of NRCAM in a completely randomized design as factorial experiment. In this study we found some salt tolerant cells among cultured calli of three soybean cultivars (KW 506, B.P. and L. 17), using this approach. We selected those cells after irradiation of calli with Gamma ray and various concentration of salt in callus weight increase (w2-WI), callus weight increase ratio [(w2-w1)/w1)] and the percentage of necrotic calli percentage. Among different doses of Gamma ray, "10 Guy" was the best, and decreased necrotic calli percentage. Despite of somaclonal variation, irradiation treatments affected the creation of tolerance trait in calli, so that a few tolerant calluses obtained up to I% of salt (NaCI).