با استفاده از الكتروفورز پروتيين ها و پلي مورفيسم ايزوزيم استراز CAMMELLIA SINENSISL ارزيابي فاصله ژنتيكي ارقام چاي

Estimation of genetic distance among Tea varieties using protein electrophoresis and polymorphism for esterase

Seed storage proteins, leaf soluble proteins and an activity of esterase were studied in 12 tea varieties. Protein bands and enzyme bands were investigated by SDS - PAGE and PAGE systems, respectively. A total of 47 bands were used to estimate the genetic distance among the varieties. These varieties were clustered together between around 0.1 to 0.5 level of distance and their mean genetical distance were estimated to be 0.316. Clustering of varieties were carried out based on 47 markers by simple matching and UPGMA method. Cluster analysis differentiated the 12 varieties in to 3 classes: 1) DN, 62.5, B275, 11, 24, 2) 10, 14, 19, 20, 31 and 3) 28, 100. The presence of Srilanka cultivars in one class and selected cultivars in a different class is a reflection of their dissimilarity and the existence of genetic distance between them. Of course, in each class a considerable amount of genetic variation can be observed.