و ارتباط آنها با تركيبات قندي TAMUS COMMUNIS L تعيين ميزان و پراكنش ديوزژنين و ساپونين در گياه تميس

The amounts and distribution of diosgenin and saponin and their Carbohydrate moiety of Tam us Communis L.

Diosgenin is a widely-used starting material for the semi-synthetic production of steroid drugs. It is obtained mainly from the tubers of Dioscorea composite, D.Floribunda, D.deltoidea and some species of Tam us (Dioscoraceae) in the tropical forest areas. Tamus Communis has been reported as a source of steroidal sapogenin. The plant used in this study named Twins (or Razak) was collected in Afra Tapeh forest (Ali-Abad city, Golestan Province), north of Iran. The sample of plant material was seperated into rhizome, leaves and berries. Acid hydrolyzed tissue was dried and extracted with chloroform. We used three different hydrolysis methods. Among them, method C (sulphuric acid in isopropanol) showed more diosgenin. In this study we showed, the sapogenin mixture and diosogenin concentration in rhizome is higher than other organs but there was no significant difference (P < 0.1). About the catbohydrate moiety, the TLC indicated the hexoses and pentoses in the leaves and stem, but no pentoses in the rhizome and seeds. In the rhizome, the amounts of soluble and insoluble sugars are more than other parts.