كليدواژه :
آنزيم هاي آنتي اكسيداني , دوره انبارماني , براسينواستروئيد , انگور
چكيده فارسي :
انگور يكي از مهم‌ترين ميوه هايي است كه توليد آن در كشور ما از سابقه بسيار طولاني برخوردار است. ميوه انگور به علت دارا بودن پوست نازك و بافتي نرم در برابر صدمات مكانيكي و انبارداري حساس است. در اين پژوهش تأثير تيمارهاي براسينواستروئيد روي ميوه‌هاي انگور رقم تجاري ريش بابا مورد مطالعه قرار گرفت، كه طي آن ميوه‌هاي انگور با غلظت‌هاي صفر (شاهد)، 0/75، 1/5 ميلي‌گرم بر ليتر براسينواستروئيد تيمار و سپس در دماي صفر تا يك درجه سلسيوس و رطوبت نسبي 90-85 درصد، به مدت6 هفته نگهداري شدند. نتايج نشان داد، كاربرد براسينواستروئيد خسارت سرمازدگي را كاهش داد. طي دوره انبار ماني، اسيديته قابل تيتراسيون و مواد جامد محلول ميوه‌ها روند كاهشي داشته در صورتي كه مقدار pH، اسيداسكوربيك و فعاليت آنزيم‌هاي آنتي‌اكسيداني (سوپراكسيد ديسموتاز وآسكوربات پراكسيداز) اين ميوه‌ها روند افزايشي را نشان دادند. به‌طوركلي ميوه‌هاي تيمار شده با 1/5 ميلي‌گرم بر ليتر براسينواستروئيد، بالاترين كيفيت ظاهري و كمترين خسارت سرمازدگي بودند.
چكيده لاتين :
Background and Objectives
Grapes are generally grown in different regions of Iran. Quality of table grapes is usually considered as a combination of appearance and flavor during shelf-life. Brassinosteroids are considered to be a class of plant polyhydroxysteroids and have been recognized as a new kind of phytohormones that play an essential role in plant development. Researchers studied the brassinosteroids levels in cucumber through a chemical genetics approach and found that BR levels were positively correlated with the tolerance to cold stresses. The objectives of this study were to evaluate the effects of BR on chilling d, lipid peroxidation content, and the induction of antioxidant enzymes, such as ascorbate peroxidase and superoxide dismutasein “Rish Baba” grape fruit during storage at 0-1 °C.
Materials and methods
Grape fruits were harvested and transported to the laboratory on the same day. The harvested fruits were washed, and dried in air. Grape fruits were treated with 0 (control), 0.75 and 1.5 milligram per liter brassinosteroid for 5 min and then stored at 0-1°C, 85-90 % relative humidity for 6 weeks. Characteristics such as chilling injury, ion leakage, lipid peroxidation, and activity of antioxidant enzymes were evaluated.
Results
Results showed that brassinosteroid significantly reduced the chilling injury, ion leakage, lipid peroxidation and hydrogen peroxide, in treated fruits, compared to control. According to these results, pH and ascorbic acid of treated and controlled fruits increased during storage while titratable acidity of fruits decreased. As for brassinosteroid treatment, the pattern of mentioned changes reduced lower than control. So, the activity of antioxidant enzymes of fruits treated with brassinosteroid highly increased compared to control during storage. Fruits treated with 1.5 mgL-1 brassinosteroid showed the best effect with lowest chilling injury.
Discussion
In the present study, the plant hormone BR was applied and the results indicated that BR significantly reduces CI of grape fruits during storage at 0-1 °C. Our finding was consistent with previous reports that exogenous application of BR is effective in protecting seedlings of rice, maize and cucumber against cold stress. CI occurrence is often accompanied by oxidative damage, which can be followed through lipid peroxidation content, since it is a final product of lipid peroxidation. In this study, there was a continuous increase in fresh tissue lipid peroxidation content in all fruits, but the application of BR significantly delayed the increase of lipid peroxidation. Moreover, the change in membrane permeability showed trends similar to lipid peroxidation content; that is, fresh tissue H2O2 content increased with storage duration, but BR markedly delayed the increase. BR has been considered to be involved in a network of interacting signal transduction pathways, which regulate defense responses to abiotic stress. When horticultural crops are exposed to severe abiotic stresses, including cold stress, large amounts of intracellular ROS are generated. The detoxification of ROS is dependent on antioxidant enzymes such as superoxide dismutase. The increase in these enzymes’ activity contributes to the adaptation of plants to cold stress and ameliorates oxidative damage such as lipid peroxidation and H2O2 content.
