بررسي مستمر سلامتي و عدم تاييد وجود بيماري نكروز هماتوپويتيك عفوني (IHN)در مولدين قزل آلاي رنگين كمان (Oncorhynchus mykiss)در حومه ياسوج به وسيله روش هاي ملكولي (RT-PCR) و كشت سلولي در طي چهار سال 1394-1391.

ويروس IHNV Infectious Hematopoietic Necrosis) (Virus كه به طور خلاصه به آن IHN مي گويند از جمله عوامل عفونت زاي مسري و مضر در قزل آلا است و سبب نوعي بيماري مهلك به عنوان "نكروز هماتوپويتيك عفوني" يا بيماري IHN مي شود (مخير، 1389؛Eiras et al., 2008 ). شايد بتوان معادل فارسي اين بيماري را "عفونت بافت خونساز يا عفونت امحاگر بافت خونساز" ناميد.

IHNV (Infectious Hematopoietic Necrosis Virus) is one of the most important pathogens in cultured rainbow trout (Oncorhynchus mykiss). The virus causes the disease that affects the hematopoietic tissue and blood vessels of fish, especially in larvae and fingerling stages. The disease is highly contagious and causes mass fish casualty (up to 95%). Therefore, continuous health monitoring of fish farms is necessary. The objectives of the present study were to identify and track the probable presence of the virus in fish farm in Dasht-e-Rome, Kohgiluyeh and Boyer-Ahmad province, Iran. Samples were prepared from the liver, spleen, kidney, egg, sperm and larvae of the adult fish and broodfish during four years from 2012 to 2016. About one percent of the broodstock population was used for sampling (1%×3000 broodfish×4years=120fish). In order to detect the presence of IHN-virus, samples were sent to a laboratory in frozen condition. After preparation of samples, they were inoculated with two types of cell line (BF2 and EPC) and examined by the molecular methods (Reverse Transcriptase-PCR) to detect the presence of IHN virus. The results showed that all samples were free from the IHN virus and the frequency of virus was 0% in all examined samples.