مطالعه تاثير اسيد فوليك بر برخي از شاخص هاي اسپرم متعاقب القا ايسكمي بازخونرساني در بيضه موش صحرايي

The effect of folic acid on sperm parameters on testicular ischemia-reperfusion injury in rat

ايسكمي ـ بازخونرساني بازگشت مجدد خون به بافت ايسكميك مي باشد. هدف از اين مطالعه بررسي تاثير تجويز اسيد فوليك بر عملكرد بيضه متعاقب القا ايسكمي ـ بازخونرساني در موش صحرايي بالغ مي باشد. اين تحقيق روي 36 سر موش صحرايي نر بالغ نژاد ويستار در محدوده سني 300-250 گرم صورت گرفت. موشها به طور تصادفي به 6 گروه 6تايي تقسيم شدند: گروه كنترل: كه هيچ دارويي دريافت نكردند گروه شم: هيچ دارويي دريافت نكرده و هر روز به حجم مساوي ساير گروه ها آب مقطر به صورت خوراكي دريافت كردند. گروه IR: كه تا زمان ايجاد IR هيچ دارويي دريافت نكرد و بعد از 1 هفته تحت عمل جراحي جهت ايجاد ايسكمي قرار گرفت. گروه چهارم، پنجم و ششم: 1 هفته قبل از ايجاد ايسكمي اسيد فوليك به ترتيب (2، 5 و10ميلي گرم/ كيلوگرم)به صورت روزانه گاواژ شد و بعد از اتمام 1 هفته تحت عمل جراحي جهت ايجاد ايسكمي قرار گرفت. بعد از آسان كشي موشها، قسمت دم اپيديديم خارج شد و بلافاصله در داخل 5 سي سي محيط HBSS قرار گرفت و به مدت 20 دقيقه در دماي 37 درجه سانتي گراد بر روي hot plate اگرمخانه گذاري شد و بعد از آن ارزيابي متغيرهاي اسپرم (شمارش تعداد اسپرم و حيات اسپرم و تحرك اسپرم) انجام شد. نتايج آماري مربوط به درصد حركت پيشرونده اسپرم، درصد حيات اسپرم و غلظت اسپرم در واحد حجم بين گروه درماني اسيد فوليك اختلاف معني داري(p<0.05) را نسبت به گروه IR نشان مي داد. براساس نتايج به دست آمده به نظر مي رسد كه اسيـد فوليـك مي تواند اثرات مناسب و قابل قبولي در پيشگيري از آسيب ايسكمي ـ بازخونرساني بيضه موش صحرايي داشته باشد.

Ischemia reperfusion definition is the return of blood to ischemia tissue. In this study, the effects of folic acid as a drug, on testicular function in adult rats were studied after induction ischemia/reperfusion. In this study, 36 male Wistar rats, the weight range between 250-300gr, were selected and divided into 6 equal groups randomly. The Control group: The Group has not received any medication and every day the same volume of distilled water was administered like other groups and after a week the abdominal cavity was opened and the left testicle was removed. The Sham group: The group has not received any medication and every day the same volume of distilled water were administered like other groups and after a week the abdominal cavity was opened and after removal of testicle from the scrotum and replacing, the abdominal cavity reclosed. I/R group: The group has not received any medication until indication of ischemia/reperfusion and every day the same volume of distilled water were administered like other groups and after 1 week the abdominal cavity was opened, and left testicular vein and artery was constricted for 1 hour thus the experimental ischemia reperfusion was induced and after 24 hour, the abdomen was opened and the left testis along with the left epididymis was extracted. 4th,5th,6th group: these groups were administered orally with (2, 5 & 10 mg/kg) of folic acid for 1 week, the abdominal cavity was opened, and left testicular vein and artery was constricted for 1 hour thus the experimental ischemia reperfusion was induced and after 24 hours the abdomen was opened and the left testis along with the left epididymis was extracted. After the rats were euthanized the epididymis were removed and then by scissors were cut into small pieces to allow sperm comfortable out there and immediately placed in 5 ml HBSS environment and for 20 minutes on a hot plate was incubated at 37°C and then evaluate sperm parameters (sperm count and sperm vitality and motility) were performed. The data were statistically analyzed and statistics on the percentage of progressive motility, sperm viability and sperm concentration per unit volume of folic acid showed significant difference (p<0.05) compared to I/R group. Based on the results obtained, it seems Folic acid can behave appropriate and acceptable effects to Prevention of Ischemia-reperfusion injury in rat testis.