مطالعه مراحل تكوين دانه گرده در Alcea aucheri (Boiss) Alef

Study on the developmental stages of Alcea aucheri (Boiss) Alef

در اﻳﻦ ﭘﮋوﻫﺶ تكوين بساك و ميكروسپورزايي ﮔﻴـﺎه ختمي جنوبي (Alcea aucheri) متعلق به تيره Malvaceae با روش‌هاي متداول برش‌گيري و توسط ميكروسكوپ نوري مورد تحقيق و بررسي قرار گرفت. ﮔـﻞﻫـﺎ وﻏﻨﭽـﻪﻫـﺎي اين گياه درﻣﺮاﺣﻞ ﻣﺨﺘﻠﻒ ﻧﻤﻮ ﺑﺮداﺷﺖ ﺷﺪه، در FAA ﺗﺜﺒﻴﺖ و ﺳﭙﺲ در اﻟﻜﻞ 70% ﻧﮕﻬﺪاري ﺷﺪﻧﺪ. ﻧﻤﻮﻧﻪﻫﺎ ﭘﺲ از آﻣﺎدهﺳـﺎزي و ﻗﺎﻟـﺐﮔﻴـﺮيدر ﭘﺎراﻓﻴﻦ، ﺑﺎ ﻣﻴﻜﺮوﺗﻮم ﺑﺮشﮔﻴﺮي ﮔﺮدﻳﺪ. رﻧﮓ‌آﻣﻴﺰي ﺑﺎ ﻫﻤﺎﺗﻮﻛﺴﻴﻠﻴﻦ-ائوزين اﻧﺠﺎم ﮔﺮﻓﺖ. ﺑﺮاﺳﺎس ﻧﺘﺎﻳﺞ ﺣﺎﺻﻞ از اﻳﻦ ﺑﺮرﺳﻲ ﺗﻜـﻮﻳﻦدﻳﻮاره ﺑﺴﺎك از ﻧﻮع دوﻟﭙﻪاي اﺳﺖ و از از ﻳﻚ ﻻﻳﻪ اﭘﻴﺪرم، ﻻﻳﻪ ﻣﻜﺎﻧﻴﻜﻲ، ﻳﻚ ﻻﻳـﻪ ﻣﻴـﺎﻧﻲ و ﻳـﻚ ﻻﻳـﻪ ﺗﻐﺬﻳـﻪﻛﻨﻨـﺪه(ﺗﺎﭘﻲ) ﺗﺸﻜﻴﻞ ﺷﺪه اﺳﺖ. ﺗﺎﭘﻲ از ﻧﻮع آميبي اﺳﺖ، ميوز با سيتوكينز همزمان دنبال مي‌شود و بطور غالب تترادهاي چهار وجهي توليد مي‌شود.

In this study, anther wall development and microsporogenesis were examined in Alcea aucheri (Malvaceae) microscopicllay using the common histological procedures. The flowers and buds, in different developmental stages, were collected from the natural habitats, fixed in FAA, stored in ethanol 70%, embedded in paraffin wax and sliced at 7-10 µm with a microtome. Staining was carried out with Hematoxylin and Eosine. According to results of this study, anddevelopment of anther wall is of dicotyledounstype and composed of one-layered epidermis, an endothecium, one middle layer and tapetum. Tapetum layer is of the amoeboid type. The meiosis division follows by simultaneous cytokinesis, producing tetrahedral tetrads, frequently.