ارزيابي اثر حفاظتي روغن سياه دانه بر بافت تخمدان در موش هاي تيمار شده با نانو ذرات نقره

Evaluation of the protective effect of Nigella sativa oil on ovarian follicles in mice treated with silver nanoparticles

هدف: هدف از‌ اين مطالعه بررسي اثر روغن سياه دانه به‌عنوان يك آنتي‌اكسيدان كارآمد، بر بافت تخمدان در موش‌هاي بالغ تيمار‌شده با نانو ذرات نقره بود. مواد ‌و ‌روش‌ها: در اين مطالعه موش‌هاي ماده بالغ نژاد NMRI به 4 گروه (6=n): كنترل، نانوذرات نقره (300 ميلي‌گرم بر كيلوگرم در روز)، عصاره سياه دانه(5 ميلي ليتر بر كيلوگرم در روز) و نانو ذرات نقره + عصاره سياه دانه تقسيم شد. تيمار دهاني تا 30 روز انجام گرفت.‌ بعد ‌از‌ تيمار، تخمدان‌ها خارج و براي ارزيابي‌ هيستولوژيكي پردازش شد. پارامترهاي مورفومتريك بافت تخمدان با استفاده از تكنيك استريولوژي تخمين زده شد. داده‌ها با استفاده از روش آناليز واريانس يك‌طرفه ارزيابي شد و (0/05

Aim: the effect of Nigella sativa oil (NSO) as an efficient antioxidant on ovarian follicles following treatment with Silver Nanoparticles (SNP) in adult mice. Material and methods: Twenty-four adult NMRI mice, weighing 27-30g were used in the present experiment. The animals were kept under optimum conditions of temperature (At 21± 2 ° C and ambient lighting conditions 12 hours of darkness and 12 hours of light) humidity and maintained on standard pellet diet with adequate water. They were divided into four groups of (six mice per group): control; Silver Nanoparticles (300 mg/kg/day, orally), Nigella sativa oil (5 ml/kg/day, orally) and finally Silver Nanoparticles plus Nigella sativa oil. After 30 days, the mice were sacrificed and the ovary tissues were separated. The results were analyzed using one-way ANOVA and Tukey’s test, and the means were significantly different at P<0.05. Results: The results of this study showed that, the mean total volume of ovary and the number of primordial, primary, secondary and graph follicles significantly decreased in the Silver Nanoparticles group compared with the control group (p<0.05). Nigella sativa oil significantly increased the above parameters in the SNP +NSO group compared to silver nanoparticles group (p<0.05). Furthermore, the number of follicles and total volume of ovary significantly increased in the Nigella sativa oil treated mice compared to the control ones (p<0.05) (Table 1). Conclusion: So it is not far-fetched that this oil has improved the factors in our study.