Cytotoxic Effect of Hydroalcoholic Extract from Satureja bachtiarica Bunge on Hela Cancer Cells Line

Background & Aim: Cervical cancer is the most common female genital cancer and the second most common cancer in women in the world. Since the herbal ingredients have been used for centuries to treat cancer, purpose of the present study was to determine the cytotoxic effect of hydroalcoholic extract from Satureja bachtiarica Bunge on Hela cancer cells line. Experimental: Hela cells line and natural fibroblast cells line were cultured in RPMI 1640 and DMEM Containing fetal bovine serum and antibiotics, respectively. The cells were exposed to different doses of hydroalcoholic extract of Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25, and 2.5 mg/ml) and were incubated for 24, 48 and 72 hr, respectively. After the incubation period, the modified MTT colorimetric test was used in order to determine cell toxicity of the extract. Results: The results of MTT test Showed that hydroalcoholic extract of Satureja bachtiarica Bunge has dose-dependent anti-cancer effect on Hela cancer cells, so that by increasing the concentration of extract and incubation in 72h, the most percentage of cell death was observed (p < 0.05). The extract of plant didn’t show any significant cytotoxicity on natural fibroblast cell line. Therefore, it seems that its compounds will be applied in treating cancer through more researches. Recommended applications/industries: Considering the low side effects of herbal medicines, especially antioxidants including Satureja bachtiarica Bunge can be used to treat cancer with drug therapy.