مقايسه ميزان بيان miR-126 در بافت سرطان سينه و بافت نرمال و ارتباط آن با سن افراد در جمعيت ايران

The comparison of the expression of miR-126 in breast and normal tissue and the association between these data and age of Iranian population

زمينه و هدف: سرطان پستان، شايع‌ترين سرطان در ميان زنان است. miRNA ها دسته‌اي از RNA هاي غير كدكننده هستند كه نقش مهمي در كنترل بيان ژن در سطح پس از رونويسي ايفا مي‌كنند. miR-126 يك miRNA اينتروني است كه در ژن EGFL7 قرار دارد. miR-126 در سرطان سينه به‌عنوان سركوب‌كننده‌ي تومور بوده و ميزان بيان آن كاهش مي‌يابد. هدف اين مطالعه بررسي ميزان بيان miR-126 در بافت توموري و نرمال سرطان سينه و ارتباط آن با سن افراد در جمعيت ايران مي‌باشد. روش بررسي: بررسي وضعيت بيان miR-126 در بافت‌هاي نرمال و سرطاني پستان توسط روش Real Time RT-PCR سنجيده و سپس ارتباط بيان اين miRNA با سن افراد محاسبه مي‌شود. ژن U6 به‌عنوان ژن كنترل انتخاب گرديد. جهت آناليزهاي آماري از روش t مستقل و ANOVA استفاده شد. يافته‌ها: نتايج نشان مي‌دهد كه بيان miR-126 به هنگام توموري شدن كاهش‌يافته و ميانگين ميزان بيان اين miRNA در گروه‌هاي نرمال با گروه‌هاي بدخيم و خوش‌خيم متفاوت است (0/05

Background and aims: Breast cancer is the most common cancer among women. miRNAs are a class of non-coding RNAs which play an important role in control of gene expression at post-transcriptional level. miR-126, an intronic miRNA derived from EGFL7 gene. miR-126 has been revealed to down-regulate and play a tumor suppressive role in breast tumors. The aim of this study was to assess the expression of miR-126 in breast and normal tissue and an association between these data and age of Iranian population. Methods: In this study, expression of miR-126 was assessed in cancer specimen and normal breast tissues by Real Time RT-PCR. Then, the association between expression of miR-126 and age were calculated. U6 gene was selected as the control. Data were analyzed statstically by ANOVA and t- test. Results: Data indicated that the expression of miR-126 was significantly down-regulated in tumor status. Also, the expression of miR-126 had a significantly difference between normal and malignant and benign tumor. (P<0.05). In addition, there was a relationship between age and the expression of miR-126, and the age of the patients who had a low expression of miR-126 was less than the age of the patients that had the high expression of miR-126. Conclusion: The results shows that miR-126 expression decreases in tumor status. In addition, a significant correlation between expression of miR-126 and age of patients was observed