بررسي تاثير مصرف داروي ضد صرع لاموتريژين، بر ميزان عملكرد هورمون هاي توليدمثلي در رت هاي نر

The Effect of Antiepileptic Drug of Lamotrigine, on the Function of Reproductive Hormones in Male Rats

مقدمه و هدف: لاموتريژين از جمله داروهاي مورد استفاده در درمان صرع است. در پژوهش حاضر اثرات داروي لاموتريژين بر ميزان عملكرد هورمون هاي محور هيپوتالاموسي- هيپوفيزي، گنادي در رت هاي نر مورد بررسي قرار گرفت. مواد و روش ها: چهل سر رت نر ويستار به 5 گروه (8=n) كنترل، شاهد كه حلال دارو بميزان 2/ 0 ميلي ليتر و سه گروه تجربي لاموتريژين با مقادير 100 ، 200 و 400 ميلي گرم به ازاي هر كيلوگرم وزن بدن به صورت خوراكي طي مدت 14 روز تقسيم شدند. نمونه هاي خوني از قلب تهيه و غلظت سرمي هورمون هاي FSH، LH، تستوسترون و نيز وزن بدن و بيضه هاي جدا شده در همه گروه ها اندازه گيري شد. يافته ها: مقادير سرمي LH تغيير معني داري در گروه هاي تجربي نسبت به گروه كنترل و شاهد نشان ندادند، اما در گروه تجربي دريافت كننده دوز 400 ميلي گرم به ازاي هر كيلوگرم وزن بدن ميزان هورمون تستوسترون و FSH كاهش معني داري (0/05

Introduction: Lamotrigine is one of the never anti-epileptic drug. In this study the effects of lamotrigine have been observed on serum concentration of LH (Luteinizing hormone)، FSH (Follicle-stimulating hormone)، testosterone ،body and testis weight in male rat. Methods: The animal used in this experiment were 40 adult male rat from wistar race which were divided in to 5 group of 8.consisting of control group which received nothings، Sham group which received 0.2 ml distilled water via oral. Experimental group which received 100، 200، 400 mg/kg lamotrigine via oral after 14th day body weight were measured in all group and then the blood sample was taken from heart and concentration of LH.FSH، testosterone was measured. In addition the testis were separated and testis weight were measured in all group. Results: The result show that concentration of LH in experimental group did not show significant difference in compared with control group but in experimental group received 400mg/kg of lamotrigine show a significant decrease in concentration of FSH and testosterone in comparison with control group .In addition lamotrigine had effect and testis weight in middle and high dose was reduce. Conclusion: Lamotrigine، an antiepileptic drug، reduced reproductive activity by inhibiting of hypothalamic-pituitary-gonadal axis in adult male rats.