Title of article :
Integrated Structural Analysis of the Human Nuclear Pore Complex Scaffold
Author/Authors :
Khanh Huy Bui، نويسنده , , Alexander von Appen، نويسنده , , Amanda L. DiGuilio، نويسنده , , Alessandro Ori، نويسنده , , Lenore Sparks، نويسنده , , Marie-Therese Mackmull، نويسنده , , Thomas Bock، نويسنده , , Wim Hagen، نويسنده , , Amparo Andrés-Pons، نويسنده , , Joseph S. Glavy ، نويسنده , , Martin Beck، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2013
Pages :
11
From page :
1233
To page :
1243
Abstract :
The nuclear pore complex (NPC) is a fundamental component of all eukaryotic cells that facilitates nucleocytoplasmic exchange of macromolecules. It is assembled from multiple copies of about 30 nucleoporins. Due to its size and complex composition, determining the structure of the NPC is an enormous challenge, and the overall architecture of the NPC scaffold remains elusive. In this study, we have used an integrated approach based on electron tomography, single-particle electron microscopy, and crosslinking mass spectrometry to determine the structure of a major scaffold motif of the human NPC, the Nup107 subcomplex, in both isolation and integrated into the NPC. We show that 32 copies of the Nup107 subcomplex assemble into two reticulated rings, one each at the cytoplasmic and nuclear face of the NPC. This arrangement may explain how changes of the diameter are realized that would accommodate transport of huge cargoes.
Journal title :
CELL
Serial Year :
2013
Journal title :
CELL
Record number :
1022025
Link To Document :
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