Liquid chromatographic determination of taxol and related derivatives using a new polyfluorinated reversed-phase column

Taxol (paclitaxel) and 5 of its commonly known derivatives (cephalomannine, 7-epi-10-deacetyltaxol, 10-deacetyltaxol, 10-deacetylbaccatin III, baccatin III) were separated by a liquid Chromatographic method using a new commercially available polyfluorinated reversed-phase column, known as Fluofix®. The assay achieved good resolution with the exception of cephalomannine and 7-epi-10-deacetyltaxol. An injectable formulation of taxol was validated using this method. The results were consistent and the procedure could be used for quality control or purity determinations of taxol in bulk and pharmaceutical dosage forms.